Leishmania donovani: Thionins, plant antimicrobial peptides with leishmanicidal activity

被引:43
作者
Berrocal-Lobo, Marta [1 ,2 ]
Molina, Antonio [1 ]
Rodriguez-Palenzuela, Pablo [1 ]
Garcia-Medo, Francisco [1 ]
Rivas, Luis [2 ]
机构
[1] Univ Politecn Madrid, Dept Biotecnol ETSI Montes, Ctr Biotecnol & Genom Plantas, INIA, Madrid 28223, Spain
[2] Ctr Invest Biol, E-28040 Madrid, Spain
关键词
Leishmania; Plant antimicrobial peptide; Thionin; Defensin; Snakin; Lipid transfer protein;
D O I
10.1016/j.exppara.2009.03.019
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The leishmanicidal activity of plant antibiotic peptides (PAPs) from the principal families, such wheat thionins, a barley lipid transfer protein and potato defensins and snakins were tested in vitro against Leishmania donovani. Only thionins and defensins were active against this human pathogen at a low micromolar range of concentrations. Thionins resulted as the most active peptides tested until now. They collapsed ionic and pH gradients across the parasite plasma membrane together with a rapid depletion of intracellular ATP without affecting mitochondrial potential. Hence the lethal effect of thionins was mostly associated to permeabilization of the plasma membrane leading to an immediate death of the parasite. The present work is the first evidence for leishmanicidal activity in plant peptides. Future prospects for their development as new antiparasite agents on human diseases are considered. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:247 / 249
页数:3
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