Genotypes Identification in Iranian Morus alba L. Populations Using Inter-simple Sequence Repeat Markers

被引:4
作者
Askari, Mehry [1 ]
Talebi, Seyed Mehdi [1 ]
Matsyura, Alex [2 ]
机构
[1] Arak Univ, Dept Biol, Fac Sci, Arak 3815688349, Iran
[2] Altai State Univ, Barnaul, Russia
来源
IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A-SCIENCE | 2021年 / 45卷 / 01期
关键词
Infraspecific variation; ISSR; Genetic diversity; Morus alba; Population structure; GENETIC DIVERSITY; MOLECULAR CHARACTERIZATION; MULBERRY GENOTYPES; ISSR; RAPD; DIFFERENTIATION; DIVERGENCE; CULTIVARS; LAMIACEAE; INFERENCE;
D O I
10.1007/s40995-020-01000-4
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Morus alba L. (Mulberry) is an economic and medicinal tree, originated from the Himalaya region and gradually spread all over the world. Most of the recently developed mulberry varieties have similar narrow genetic base and limited capacity against environmental stress and pathogens. To enhance the species resistance, we need to increase genetic diversity of its varieties using higher genotypes differentiation in breeding programs. We investigated genetic diversity and structure of some mulberry Iranian populations to register different genotypes, which could be implemented in further genetic breeding programs. For this purpose, the nuclear genome of ten populations (4-7 individuals per each population) were extracted using the CTAB modified method and amplified using 30 ISSR primers. Genetic diversity parameters, polymorphism indexes and population structure were detected using GenAlex v. 6.4, GenoDive v. 2.0, PopGene v. 1.32 and Structure. We detected that the number of different alleles and polymorphic bands together with genetic diversity parameters varied among the populations. The results of AMOVA demonstrated the significant genetic interpopulation variations. Our research outputs were consistent with the high G(ST) and H-T amounts and low rate of N-m and H-s, which indicating high genetic differentiation of the populations. These results suggested that genetic drift created high genetic differentiation among some populations. We determined the optimal value of K (6) by structure analysis and K-means clustering. This was supported by six detected genotypes among mulberry populations based on the UPGMA tree of Nei's genetic distance, MDS plot, structure analysis and neighbor-net diagram. These genotypes have high genetic diversity, and they should be considered in prospective breeding programs to develop the mulberry varieties with improved yield and quality, and enhanced tolerance toward adverse environmental stress and diseases.
引用
收藏
页码:91 / 102
页数:12
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