Hardware Integration and Software Design of Automated Nucleic acid extraction System based on Magnetic Bead Separation Method

被引：0

作者：

Yu, Yang ^{[1
]}

Dou, Yongjie ^{[1
]}

Li, Qian ^{[1
,2
]}

Zhao, Xiaoli ^{[3
]}

Xu, Jiansheng ^{[1
]}

Deng, Congliang ^{[3
]}

Wang, Ming ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Elect Engn, Beijing, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

[3] Beijing Inspect & Quarantine Ctr, Plant Lab, Beijing, Peoples R China

来源：

PROCEEDINGS OF THE 3RD INTERNATIONAL CONFERENCE ON MATERIAL, MECHANICAL AND MANUFACTURING ENGINEERING | 2015年 / 27卷

关键词：

nucleic acid extraction; magnetic bead; Plant viral RNA; RNA; DNA;

D O I：

暂无

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

This paper presents a system designed for automated nucleic acid extraction machine based on magnetic bead separation method. A hardware platform has been established which consists of motor controlling module, temperature controlling module and a computer; also, we designed the system software to achieve data acquisition, calculation, display, management and acid extraction control. The sample temperature achieved the required value within 13 min, and the accuracy of temperature control can be up to about +/- 1 degrees Celsius, when the sample amount is the maximum capacity of the sample matrix. And each nucleic acid purification experiment time is controlled within 60min. Plant viral RNA extraction experiments have been done on this platform. The automatic nucleic acid extraction instrument can be successfully used for the purification of DNA and RNA, and achieved very satisfactory experimental results.

引用

页码：1398 / 1403

页数：6