Single-molecule identification by spectrally and time resolved fluorescence detection

被引：50

作者：

Prummer, M ^{[1
]}

Hübner, CG ^{[1
]}

Sick, B ^{[1
]}

Hecht, B ^{[1
]}

Renn, A ^{[1
]}

Wild, UP ^{[1
]}

机构：

[1] ETH Zurich, CH-8092 Zurich, Switzerland

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 03期

关键词：

D O I：

10.1021/ac991116k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method to identify single molecules rapidly and with high efficiency based on simple probability considerations is proposed. In principle, any property of a detected photon in a single-molecule fluorescence experiment, e.g., emission wavelength, arrival time after pulsed excitation, and polarization, can be analyzed within the framework of the outlined methodology. Monte Carlo simulations show that less than 500 photons are needed to assign an observed single molecule to one out of four species with a confidence level higher than 99.9%. We show that single dye molecules of four different dyes embedded in a polymer film can be identified with time-correlated single-photon counting spectrally resolved in two channels.

引用

页码：443 / 447

页数：5

共 14 条

[1] Time-resolved detection and identification of single analyte molecules in microcapillaries by time-correlated single-photon counting (TCSPC) [J].