Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor

被引：5

作者：

Gao, Jiangtao

Qin, Song ^{[1
]}

Zhang, Yicheng

机构：

[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

CHEMICAL ENGINEERING JOURNAL | 2006年 / 122卷 / 1-2期

关键词：

gametophyte cells; HBsAg; Laminaria japonica; pH; process conditions; stirred-tank bioreactor;

D O I：

10.1016/j.cej.2006.05.004

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 degrees C and 200 rpm. Optimal incident light intensities depended on the initial cell densities. (c) 2006 Elsevier B.V. All fights reserved.

引用

页码：11 / 14

页数：4

共 13 条

[1] DRUGS FROM SEAWEEDS [J].