Characterization of Elongation Factor Tu of Mycoplasma ovipneumoniae

被引：1
|
作者：
Zhang, Xuan [1 ]
Chu, Yue-feng [1 ]
Zhao, Ping [1 ]
Gao, Peng-cheng [1 ]
He, Ying [1 ]
Wang, Nu [1 ]
Lu, Zhong-xin [1 ]
机构：
[1] Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Key Lab Epizoot Dis Grazing Anim, Minist Agr,Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China
来源：
PAKISTAN VETERINARY JOURNAL | 2013年 / 33卷 / 04期
关键词：
Antigenicity; EF-Tu; Mycoplasma ovipneumoniae; IMMUNOPROTEOMIC IDENTIFICATION; BIGHORN SHEEP; PROTEINS; ANTIGENS; CELLS;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Mycoplasma ovipneumoniae is considered as an important pathogen of small ruminants, but its antigenic proteins are not well known so far. In this study, we cloned the EF-Tu gene of M. ovipneumoniae and analyzed the molecular features of the gene and its coding protein for the first time. The gene was then expressed in E. coli and the antigenicity of the coding protein was evaluated as well. The EF-Tu gene of M. ovipneumoniae is 1209 bp in length, encodes 402 amino acids, and shares the highest DNA sequence identity of 87.5% and deduced amino acid sequence identity of 97.8% with those of M. hyopneumoniae, respectively. The recombinant EF-Tu protein can react with the polyclonal antiserum of M. ovipneumoniae and can induce humoral immune responses in mice, which indicated that the EF-Tu may be used as a candidate protein in developing the technologies to control the disease. (C) 2013 PVJ. All rights reserved
引用
收藏
页码：515 / 519
页数：5
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