ATG5 expression induced by MDMA (ecstasy), interferes with neuronal differentiation of neuroblastoma cells

被引：14

作者：

Chae, Myounghee ^{[3
]}

Rhee, Gyu-Seek ^{[4
]}

Jang, Ik-Soon ^{[3
]}

Kim, Kwangsoo ^{[3
]}

Lee, Ji-Hae ^{[2
]}

Lee, Seung-Yeul ^{[2
]}

Kim, Minjung ^{[2
]}

Yang, Junyoung ^{[4
]}

Park, Junsoo ^{[1
]}

Lee, Seung-Hoon ^{[2
]}

机构：

[1] Yonsei Univ, Div Biol Sci & Technol, Wonju 220100, South Korea

[2] Yong In Univ, Dept Biol Sci, Yongin 449719, South Korea

[3] Gwangju Ctr, Korea Basic Sci Inst, Kwangju 500757, South Korea

[4] Korea Food & Drug Adm, Natl Inst Toxicol Res, Reprod & Dev Toxicol Div, Seoul 122704, South Korea

来源：

MOLECULES AND CELLS | 2009年 / 27卷 / 05期

关键词：

Atg5; autophagy; MDMA; neuron; PC12; SH-SY5Y; CASPASE-3; ACTIVATION; NEURITE OUTGROWTH; INDUCED APOPTOSIS; DNA-DAMAGE; AUTOPHAGY; PROTEIN; DEATH; SURVIVAL; ROLES;

D O I：

10.1007/s10059-009-0075-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The amphetamine derivative 3, 4-methylenedioxymethamphetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.

引用

页码：571 / 575

页数：5

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