Construction of a recombinant B-cell epitope vaccine based on a Der p1-derived hypoallergen: a bioinformatics approach

被引：15

作者：

Fanuel, Songwe ^{[1
,2
]}

Tabesh, Saeideh ^{[4
]}

Mokhtarian, Kobra ^{[5
]}

Saroddiny, Esmaeil ^{[1
]}

Fazlollahi, Mohammad Reza ^{[2
]}

Pourpak, Zahra ^{[2
]}

Falak, Reza ^{[3
]}

Kardar, Gholam Ali ^{[1
,2
]}

机构：

[1] Tehran Univ Med Sci Int Campus IC TUMS, Sch Adv Technol Med, Dept Med Biotechnol, Tehran, Iran

[2] Univ Tehran Med Sci, IAARI, Tehran, Iran

[3] Iran Univ Med Sci, Sch Med, Dept Immunol, Tehran, Iran

[4] Univ Tehran Med Sci, Sch Publ Hlth, Dept Immunol, Tehran, Iran

[5] Shahrekord Univ Med Sci, Med Plant Res Ctr, Shahrekord, Iran

来源：

IMMUNOTHERAPY | 2018年 / 10卷 / 07期

关键词：

allergen-specific immunotherapy; allergy; Der p1; epitopes; house dust mite; tetanus toxoid; HOUSE-DUST MITE; ALLERGEN-SPECIFIC IMMUNOTHERAPY; PROTEIN; EXTRACTS; MODEL; WEB; ANTIBODIES; PEPTIDE; IMMUNE; GAMMA;

D O I：

10.2217/imt-2017-0163

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Aim: House dust mite (HDM) allergens are important elicitors of IgE-mediated allergies. This study was aimed at constructing and characterizing a recombinant fusion protein, DpTTDp, which was based on carrier-bound Der p 1-derived peptides for HDM allergen immunotherapy. Methods: Using the Immune Epitope Database (IEDB), we identified from Der p 1, a 34-mer hypoallergenic peptide. Two copies of the hypoallergen were then fused to a partial fragment of a tetanus toxoid molecule's N- and C terminus and expressed in Escherichia coli. After purification to homogeneity, the protein was evaluated for allergenicity and its ability to induce blocking antibodies upon immunization. Results: Upon immunization of mice, DpTTDp induced high levels of protective IgG-antibodies that blocked allergic patients' IgE reactivity to HDM. In addition, DpTTDp lacked relevant IgE-reactivity, induced low T-cell proliferation and IFN-gamma in peripheral blood mononuclear cells of HDM-allergic patients' sera. Conclusion: The protein represents a promising HDM-allergy immunotherapy candidate vaccine.

引用

页码：537 / 553

页数：17

共 57 条

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