Enhanced cytidine production by a recombinant Escherichia coli strain using genetic manipulation strategies

被引：15

作者：

Fang, Haitian ^{[1
,2
]}

Zhang, Chenglin ^{[2
]}

Xie, Xixian ^{[2
]}

Xu, Qingyang ^{[2
]}

Zhou, Yunjiao ^{[2
]}

Chen, Ning ^{[2
,3
]}

机构：

[1] Ningxia Univ, Coll Agr, Yinchuan, Peoples R China

[2] Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China

[3] Tianjin Univ Sci & Technol, Coll Biotechnol, Metab Engn Lab, Tianjin 300457, Peoples R China

来源：

ANNALS OF MICROBIOLOGY | 2014年 / 64卷 / 03期

关键词：

Cytidine; Escherichia coli; Fermentation; Gene knockout; pyrB-pyrA operon; COMPLETE GENOME SEQUENCE; HOMOSERINE DEHYDROGENASE; CHROMOSOMAL GENES; THYMIDINE; EXPRESSION; BIOSYNTHESIS; INACTIVATION; SYNTHETASE; DEAMINASE; MULTIPLE;

D O I：

10.1007/s13213-013-0760-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cytidine is a nucleoside molecule that is widely used as a precursor for antiviral drugs. In this study, a cytidine-producing strain Cyt18 was developed from Escherichia coli K-12 through 3-step genetic manipulation strategies. Cytidine deaminase gene (cdd) was firstly deleted from the E. coli K-12 strain to develop Cyt10. Furthermore, homoserine dehydrogenase gene (thrA) was inactivated from the Cyt10 strain to develop Cyt12, in which the intracellular aspartate concentration was expected to be increased. The recombinant plasmid pMG1105 containing an pyrB-pyrA operon from Bacillus amyloliquefaciens CYTI was constructed and was introduced into Cyt12 to obtain the Cyt18 strain. Compared to the Cyt12 strain, the cytidine production by the recombinant strain Cyt18 was increased by similar to 3-fold (722.9 mg/l vs. 249.3 mg/l).

引用

页码：1203 / 1210

页数：8

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