Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum

被引：25

作者：

Kuzmanovic, Nemanja ^{[1
]}

Pulawska, Joanna ^{[2
]}

Prokic, Andelka ^{[1
]}

Ivanovic, Milan ^{[1
]}

Zlatkovic, Nevena ^{[1
]}

Jones, Jeffrey B. ^{[3
]}

Obradovic, Aleksa ^{[1
]}

机构：

[1] Univ Belgrade, Fac Agr, Nemanjina 6, Belgrade 11080, Serbia

[2] Inst Hort Res, Ul Konstytucji 3 Maja 1-3, PL-96100 Skierniewice, Poland

[3] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2015年 / 38卷 / 06期

关键词：

Agrobacterium; Crown gall; 16S rDNA; MLSA; ANI; Taxonomy; RHIZOBIUM-RADIOBACTER; SEQUENCE-ANALYSIS; GENUS; IDENTIFICATION; DIVERSITY; DNA; TUMEFACIENS; DELINEATION; POPULATION; SPECIATION;

D O I：

10.1016/j.syapm.2015.06.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values (<92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)). (C) 2015 Elsevier GmbH. All rights reserved.

引用

页码：373 / 378

页数：6

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