Inhibitory effects of Danhong Injection and its major constituents on human cytochrome P450 enzymes in vitro

被引：12

作者：

Ye, Lin-Hu ^{[1
,2
,3
]}

Zhao, Xin-Qian ^{[1
]}

Kong, Ling-Ti ^{[2
,3
,4
]}

Wang, Li-Sha ^{[2
,3
]}

Tao, Xue ^{[2
,3
]}

Wu, Hui ^{[1
]}

He, Mei ^{[1
]}

Chang, Qi ^{[2
,3
]}

机构：

[1] First Peoples Hosp Bijie, Dept Pharm, Bijie, Peoples R China

[2] Chinese Acad Med Sci, Inst Med Plant Dev, Beijing, Peoples R China

[3] Peking Union Med Coll, Beijing, Peoples R China

[4] Bengbu Med Coll, Dept Pharm, Affiliated Hosp 1, Bengbu, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2018年 / 32卷 / 08期

关键词：

cytochrome P450; Danhong Injection; drug interactions; HPLC-MS; MS; traditional Chinese medicine; HUMAN LIVER-MICROSOMES; SALVIA-MILTIORRHIZA; ACID; IDENTIFICATION; THERAPEUTICS; DRUGS; MS/MS; RATS;

D O I：

10.1002/bmc.4250

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Danhong Injection (DHI) as a Chinese patent medicine is mainly used to treat ischemic encephalopathy and coronary heart disease in combination with other chemotherapy. However, the information on DHI's potential drug interactions is limited. The goal of this work was to examine the potential P450-mediated metabolism drug interaction arising from DHI and its active components. The results showed that DHI inhibited CYP2C19, CYP2D6, CYP3A4, CYP2E1 and CYP2C9 with IC50 values of 1.26, 1.42, 1.63, 1.10 and 1.67% (v/v), respectively. Danshensu and rosmarinic acid inhibited CYP2E1 and CYP2C9 with IC50 values of 36.63 and 75.76m, and 34.42 and 76.89m, respectively. Salvianolic acid A and B inhibited CYP2D6, CYP2E1 and CYP2C9 with IC50 values of 33.79, 21.64 and 31.94m, and 45.47, 13.52 and 24.15m, respectively. The study provides some useful information for safe and effective use of DHI in clinical practice.

引用

页数：6

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