Improvement of cephalomanine production in Taxus chinensis cells by a combination of sucrose and methyl jasmonate

被引：5

作者：

Lan, WZ ^{[1
]}

Yu, LJ ^{[1
]}

Cheng, P ^{[1
]}

Li, MY ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Wuhan 430074, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 15期

关键词：

cephalomanine; geranylgeranyl diphosphate synthase; methyl jasmonate; sucrose supplementation; Taxus chinensis;

D O I：

10.1023/A:1016269627346

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell suspension cultures of Taxus chinensis, supplemented with 25 g sucrose l(-1), produced 11 mg cephalomanine l(-1),21 g biomass l(-1) and 19 nkat geranylgeranyl diphosphate (GGPP) synthase activity g protein(-1). Supplementation of the cultures with 100 muM methyl jasmonate (MJA) produced 17 mg cephalomanine l(-1), 6 g biomass l(-1) and 78 nkat GGPP synthase activity g protein(-1). Addition of sucrose and MJA together produced 24 mg cephalomanine l(-1), 18 g biomass l(-1) and 55 nkat GGPP synthase activity g protein(-1).

引用

页码：1253 / 1255

页数：3

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