Endogenous prostaglandins regulate rat renal phospholipid 'de novo' synthesis

Rat renal papilla is the zone of the kidney enjoying the most active phospholipid metabolism and also the highest prostanlandin production. We studied the phospholipid biosynthesis and the relationship between phospholipid de novo synthesis and prostaglandin biosynthesis in rat renal papilla. Indomethacin inhibited the biosynthesis of phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine and phosphatidic acid. Exogenous PGF(2 alpha) and PGD(2) restored biosynthetic activity in the presence of indomethacin and also increased the activity of the enzymes involved in the Kennedy pathway. The decrease in phospholipid biosynthesis maintained a linear relationship with the decrease in prostaglandin biosynthesis. Moreover, esculetin, which stimulates prostaglandin synthesis, brought about a significant increase in P-32 incorporation to the three phospholipids studied. The evidence presented in this paper indicates that renal PGF(1 alpha) and PGD(2) modulate phospholipid de novo synthesis in rat renal papilla. (C) 1997 Elsevier Science B.V.