TOTAL PHENOLIC AND FLAVONIOD CONTENT AND ANTIOXIDANT PROPERTIES OF NIGELLA SATIVA L. SEEDS

被引：0
作者：
Ahmed, Adel Fahmi [1 ,2 ]
He, Nan [1 ]
Xia, Zhao-yang [1 ]
Kang, Wen-Yi [1 ,3 ]
机构：
[1] Henan Univ, Inst Chinese Mat Med, Kaifeng 475004, Henan, Peoples R China
[2] Agr Res Ctr, Hort Res Inst, Med & Aromat Plants Res Dept, Giza, Egypt
[3] Kaifeng Key Lab Funct Components Hlth Food, Kaifeng 475004, Peoples R China
来源：
CURRENT TOPICS IN NUTRACEUTICAL RESEARCH | 2018年 / 16卷 / 02期
关键词：
Antioxidant activity; DPPH assay; Macroporous Resin; Total flavonoid; Total phenolic; EXTRACTS; FLAVONOIDS; THYMOQUINONE; SOLVENT; OIL;
D O I：
暂无
中图分类号：
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号：
100403 ;
摘要：
The present study aimed to determine and compare total phenolic, flavonoid contents and antioxidant activity of Nigella sativa L. seeds crude extract and various fractions obtained through macroporous resin. Antioxidant activity were evaluated by DPPH assay. Total phenolic and flavonoid contents were determined as pyrocatechol and rutin equivalents, respectively, and correlated with antioxidant activities. Results showed that fraction with 40% ethanol exhibited the highest content of total phenolic (138.85 +/- 0.53 mg PE/g extract). Besides, the highest concentration offlavonoids (140.11 +/- 5.47mg RU/g extract) and the highest antioxidant activity DPPH assay (IC50 = 1.26 +/- 0.21 mu g/ml) were observed for fraction with 20% ethanol. Total phenolic content and total flavonoid highly correlated each other and well correlated with antioxidant activity. To conclude, macroporous resin could be a very good low cost and nontoxic technical for natural sources of antioxidant substances with high value for fractionation of N. sativa L. seeds with 20% ethanol.
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页码：147 / 153
页数：7
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