Effects of Different Temperatures on the Chemical Structure and Antitumor Activities of Polysaccharides from Cordyceps militaris

被引:47
作者
Nurmamat, Eliyas [1 ,2 ]
Xiao, Hongxing [1 ]
Zhang, Yan [3 ]
Jiao, Ziwei [2 ]
机构
[1] Northeast Normal Univ, Coll Life Sci, Changchun 130024, Jilin, Peoples R China
[2] Yili Normal Univ, Coll Biol & Geog Sci, Yining 835000, Peoples R China
[3] Tianjin Univ Sci & Technol, Sch Food Engn & Biotechnol, Key Lab Food Nutr & Safety, Minist Educ, Tianjin 300457, Peoples R China
关键词
C; militaris; extraction temperature; polysaccharides; physicochemical properties; antitumor activity; ANTIOXIDANT ACTIVITIES; BIOACTIVE POLYSACCHARIDES; ASTRAGALUS-MEMBRANACEUS; EXTRACTION METHODS; CANCER-CELLS; IN-VITRO; APOPTOSIS; ULTRASOUND; OPTIMIZATION; IMMUNITY;
D O I
10.3390/polym10040430
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
The effects of different extraction temperatures (4 and 80 degrees C) on the physicochemical properties and antitumor activity of water soluble polysaccharides (CMPs-4 and CMPs-80) from Cordyceps militaris (C. militaris) were evaluated in this study. The results of gas chromatography (GC) and high-performance gel permeation chromatography (HPGPC) showed that a higher extraction temperature could degrade the polysaccharides with 188 kDa, mainly composed of glucose, and increase the dissolution rate of polysaccharides about 308 kDa, mainly consisting of rhamnose and galactose. In addition, the CMPs displayed the same sugar ring and category of glycosidic linkage based on Fourier-transform infrared spectroscopy (FTIR) analysis, however, their invisible structural difference occurred in the specific rotation and conformational characteristics according to the results of specific optical rotation measurement and Congo red test. In vitro antitumor experiments indicated that CMPs-4 possessed stronger inhibitory effects on human esophagus cancer Eca-109 cells by inducing cell apoptosis more than CMPs-80 did. These findings demonstrated that the polysaccharides extracted with cold water (4 degrees C) could be applied as a novel alternative chemotherapeutic agent or dietary supplement with its underlying antitumor property.
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页数:12
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