FREQUENCY OF VIRULENCE GENES IN ESCHERICHIA COLI STRAINS ISOLATED FROM PIGLETS WITH DIARRHEA IN THE NORTH PARANA STATE, BRAZIL

被引:0
作者
Vidotto, Marilda C. [1 ]
de Lima, Natalia C. S.
Fritzen, Juliana T. T. [2 ]
de Freitas, Julio C.
Venancio, Merson J. [2 ]
Ono, Mario A. [2 ]
机构
[1] Univ Estadual Londrina, Dept Vet Prevent Med, CCA, BR-86051970 Londrina, Parana, Brazil
[2] Univ Estadual Londrina, Dept Ciencias Patol, BR-86051970 Londrina, Parana, Brazil
关键词
porcine Escherichia coli; virulence genes; postweaning diarrhea; toxins; EDEMA DISEASE; POSTWEANING DIARRHEA; FIMBRIAL ANTIGENS; PIGS; SEROGROUPS; PREVALENCE; ENTEROTOXINS; SWINE;
D O I
10.1590/S1517-83822009000100035
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100 Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty four patterns of virulence factor according to the different virulence genes form were found and the most frequent virulence gene pattern was F4, F18, F41, STa, STb and LT. Most of the isolates that carried genes for adhesins also harboured genes for toxins.
引用
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页码:199 / 204
页数:6
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