Isolation of Toxoplasma gondii From the Keel-Billed Toucan (Ramphastos sulfuratus) From Costa Rica

被引:11
作者
Dubey, J. P. [1 ]
Velmurugan, G. V. [1 ]
Morales, J. A. [2 ]
Arguedas, R. [3 ]
Su, C. [4 ]
机构
[1] USDA ARS, Serv Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA
[2] Univ Nacl Autonoma, Escuela Med Vet, Dept Patol, Heredia 3000, Costa Rica
[3] Zool Nacl Simon Bolivar, San Jose, Costa Rica
[4] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA
关键词
FREE-RANGE CHICKENS; GENETIC-CHARACTERISTICS; CENTRAL-AMERICA; OOCYSTS;
D O I
10.1645/GE-1846.1
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Pectoral muscles from a captive keel-billed toucan (Ramphastos sulfuratus) from Cost Rica were fed to a Toxoplasma gondii-free cat, and the cat shed oocysts. Laboratory mice fed these oocysts developed antibodies to T. gondii in their sera and T. gondii tissue cysts in their brains. The DNA extracted from the brains of infected mice was characterized using 10 polymerase chain reaction-restricted fragment length polymorphic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). The isolate designated TgRsCr1 was found to be non-clonal with Type I, II, and III alleles at different loci. This is the first isolation of T. gondii from this host.
引用
收藏
页码:467 / 468
页数:2
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