Determination of age-related changes in human soluble interleukin 2 receptor in body fluids of normal subjects as a control value against disease states

被引：19

作者：

Gotoh, Y

Okamoto, Y

Uemura, O

Mori, N

Tanaka, S

Ando, T

Nishida, M

机构：

[1] Meijo Univ, Fac Pharm, Dept Clin Pharm, Tempaku Ku, Nagoya, Aichi 4688503, Japan

[2] Komaki City Hosp, Dept Pediat, Aichi 4858520, Japan

[3] Nagoya Daini Red Cross Hosp, Dept Pediat, Showa Ku, Nagoya, Aichi 4668650, Japan

来源：

CLINICA CHIMICA ACTA | 1999年 / 289卷 / 1-2期

关键词：

soluble interleukin 2 receptors (sIL-2R); ELAST (R); human;

D O I：

10.1016/S0009-8981(99)00161-8

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A highly sensitive enzyme-linked immunosorbent assay (ELISA) system was developed for human soluble interleukin-2 receptor (sIL-2R) with an ELISA-amplification system (ELAST(R)). The sensitivity of the new method was 20-fold higher than that without the amplification. Thus very low concentrations of sIL-2R in urine can be detected. With this method, serum and urine concentrations of sIL-2R were analyzed for healthy Japanese subjects with age 1-67 years. Mean sIL-2R concentrations in both serum and urine of children were significantly higher than those of adults. However, the concentrations of children showed a progressive decline to those of adults by the age of 15 years. There was no difference in the values between males and females. The results provide a control value of sLL-2R against those in disease states such as nephrotic syndrome. Since the trends in serum and urine were found to be similar, urinary sIL-2R measurement may provide sufficient information, without measuring the blood concentration. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：89 / 97

页数：9