Molecular cloning and characterization of a cDNA encoding the rat interleukin-8 receptor

被引：6

作者：

Gobl, AE ^{[1
]}

Huang, MR ^{[1
]}

Wang, S ^{[1
]}

Zhou, YH ^{[1
]}

Oberg, K ^{[1
]}

机构：

[1] UNIV UPPSALA HOSP,DEPT CLIN IMMUNOL,S-75185 UPPSALA,SWEDEN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1997年 / 1326卷 / 02期

关键词：

RT-PCR; in situ hybridization; chemokine; G protein; gene expression;

D O I：

10.1016/S0005-2736(97)00065-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study we present the cloning, characterization and expression analysis of a cDNA encoding a rat interleukin-8 receptor (rIL-8R). A 1324 bp cDNA containing an open reading of 359 amino acids with an 86.1% overall identity with the previously characterized mouse IL-8R was isolated. Genomic DNA analysis using several restriction enzymes revealed a single band suggesting that the rIL-8R gene exists as a single-copy, which is in contrast to humans where there are two different IL-8Rs genes. Expression of rIL-8R mRNA was found in several tissues including spleen, heart, lung, liver, skeletal muscle and kidney. In brain and testis rIL-8R mRNA was not detectable. Rat IL-8R mRNA expression at the cellular level was studied in the spleen using RNA-RNA in situ hybridization and immunohistochemistry. IL-8R mRNA containing cells were predominately found in the mantle zone of the germinal center. These cells were identified as B lymphocytes using the OX-33 monoclonal antibody.

引用

页码：171 / 177

页数：7

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