The object of the research is the painting, Portrait of a Musician". Upon closer examination of the painting, we discovered three details. Two have the shape of trachea and bronchi and the third a larynx. By mov-ing the details through the program Paint X we got an image of the larynx, trachea and bronchi. The larynx is presented by thyroid and cricoid cartilage. The characteristic cartilaginous rings are distinguished on the trachea. The right and left main bronchi are also seen. In our opinion, Leonardo da Vinci reflected the elements of the respiratory system in the, Portrait of a Musician", thus emphasizing the fact that the bronchi, trachea and larynx are involved in the production of sound.