Preparation of multiwalled carbon nanotube-chitosan-alcohol dehydrogenase nanobiocomposite for amperometric detection of ethanol

被引：71

作者：

Lee, Chen-An ^{[1
]}

Tsai, Yli-Chen ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 402, Taiwan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 138卷 / 02期

关键词：

Multiwalled carbon nanotube; Alcohol dehydrogenase; Chitosan; Ethanol biosensor; ELECTROCATALYTIC OXIDATION; OXIDASE NANOBIOCOMPOSITE; MODIFIED ELECTRODES; GAS-CHROMATOGRAPHY; NADH DETECTION; BIOSENSOR; IMMOBILIZATION; GLUCOSE; BEVERAGES; GRAPHITE;

D O I：

10.1016/j.snb.2009.01.001

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric biosensor for the determination of ethanol has been constructed using a nanobiocomposite film. This film is made up of a conduit of multiwalled carbon nanotubes (MWCNTs), a chitosan (CHIT) hinder, and an alcohol dehydrogenase (ADH) function. The MWCNT-CHIT-ADH nanobiocomposite film is cast oil a glassy carbon electrode. Homogeneity of the resulting nanobiocomposite film was investigated by field emission scanning electron microscopy. Electrocatalytic activity of the MWCNT-CHIT-ADH nanobiocomposite was determined using cyclic voltammetry and amperometry. Optimum conditions of the biosensor were experimentally determined through varying several important parameters. These included working potential, Solution pH value, NAD(+) concentration, and enzyme loading. The optimized biosensor shows a sensitivity of 0.1646 AM(-1) cm(-1), all apparent Michaelis-Menten constant of 0.38 mM, and a detection limit of 0.52 mu M. The applicability of the proposed biosensor based on MWCNT-CHIT-ADH nanobiocomposite was tested by the detection of ethanol in beer, red wine, and spirit. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：518 / 523

页数：6

共 39 条

[1] Exploring the electrocatalytic sites of carbon nanotubes for NADH detection: an edge plane pyrolytic graphite electrode study [J].

Banks, CE ;

Compton, RG .

ANALYST, 2005, 130 (09) :1232-1239

[2] Electrocatalysis at graphite and carbon nanotube modified electrodes: edge-plane sites and tube ends are the reactive sites [J].

Banks, CE ;

Davies, TJ ;

Wildgoose, GG ;

Compton, RG .

CHEMICAL COMMUNICATIONS, 2005, (07) :829-841

[3] Modified electrodes for NADH oxidation and dehydrogenase-based biosensors [J].

Bartlett, PN ;

Simon, E ;

Toh, CS .

BIOELECTROCHEMISTRY, 2002, 56 (1-2) :117-122

[4] Amperometric biosensor for ethanol based on immobilization of alcohol dehydrogenase on a nickel hexacyanoferrate modified microband gold electrode [J].

Cai, CX ;

Xue, KH ;

Zhou, YM ;

Yang, H .

TALANTA, 1997, 44 (03) :339-347

[5] Electrocatalytic oxidation of NADH at an ordered carbon nanotubes modified glassy carbon electrode [J].

Chen, J ;

Bao, JC ;

Cai, CX ;

Lu, TH .

ANALYTICA CHIMICA ACTA, 2004, 516 (1-2) :29-34

[6] Amperometric ethanol biosensor based on carbon nanotubes dispersed in sol-gel-derived titania - nafion composite film [J].

Choi, Han Nim ;

Lyu, Young-Ku ;

Han, Jee Hoon ;

Lee, Won-Yong .

ELECTROANALYSIS, 2007, 19 (14) :1524-1530

[7] Highly sensitive lactate biosensor by engineering chitosan/PVI-Os/CNT/LOD network nanocomposite [J].

Cui, Xiaoqiang ;

Li, Chang Ming ;

Zang, Jianfeng ;

Yu, Shucong .

BIOSENSORS & BIOELECTRONICS, 2007, 22 (12) :3288-3292

[8] Electrochemical detection of carbohydrates at carbon-nanotube modified glassy-carbon electrodes [J].

Deo, RP ;

Wang, J .

ELECTROCHEMISTRY COMMUNICATIONS, 2004, 6 (03) :284-287

[9] Single-walled carbon nanotubes functionalized with poly(nile blue A) and their application to dehydrogenase-based biosensors [J].

Du, Pan ;

Liu, Shuna ;

Wu, Ping ;

Cai, Chenxin .

ELECTROCHIMICA ACTA, 2007, 53 (04) :1811-1823

[10] Application of conducting polymers to biosensors [J].

Gerard, M ;

Chaubey, A ;

Malhotra, BD .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (05) :345-359

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