Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering

被引：85

作者：

Galarza, Janeth I. ^{[1
]}

Gimpel, Javier A. ^{[2
]}

Rojas, Veronica ^{[3
]}

Arredondo-Vega, Bertha O. ^{[4
]}

Henriquez, Vitalia ^{[3
,5
]}

机构：

[1] Univ Estatal Peninsula Santa Elena, Fac Ciencias Mar, Lab Biotecnol, La Libertad, Ecuador

[2] Univ Chile, Ctr Biotecnol & Bioengn CEBIB, Dept Ingn Quim & Biotecnol, Santiago, Chile

[3] Pontificia Univ Catolica Valparaiso, Fac Ciencias, Inst Biol, Lab Genet & Inmunol Mol, Valparaiso, Chile

[4] Ctr Invest Biol Noroeste CIBNOR, Lab Biotecnol Microalgas, La Paz, Baja Calif Sur, Mexico

[5] Fraunhofer Chile Res Fdn, Ctr Syst Biotechnol, Santiago, Chile

来源：

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2018年 / 31卷

关键词：

COMPLEX METABOLIC PATHWAYS; PHYTOENE DESATURASE; CHLAMYDOMONAS-REINHARDTII; MICROALGAE; EXPRESSION; BIOSYNTHESIS; TRANSFORMATION; PLANTS; CHLOROPHYCEAE; CAROTENOIDS;

D O I：

10.1016/j.algal.2018.02.024

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The carotenoid biosynthesis pathway of Haematococcus pluvialis has been genetically modified to overproduce astaxanthin, a red pigment of high commercial value. The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After biolistic transformation and selection, PDS mRNA and protein were detected in transformants. Astaxanthin accumulated up to 67% higher in transformed strains than in wild-type upon induction with high light intensity and nitrogen depletion. This is the first report of plastid transformation of a microalgae with its endogenous pds nuclear gene.

引用

页码：291 / 297

页数：7

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