The Phytochemical Profile and Biological Activity of Malva neglecta Wallr. in Surgically Induced Endometriosis Model in Rats

被引:3
作者
Akkol, Esra Kupeli [1 ]
Karpuz, Busra [2 ]
Turkcanoglu, Gizem [1 ]
Cosguncelebi, Fatma Gul [1 ]
Tastan, Hakki [3 ]
Aschner, Michael [4 ]
Khatkar, Anurag [5 ]
Sobarzo-Sanchez, Eduardo [6 ,7 ]
机构
[1] Gazi Univ, Fac Pharm, Dept Pharmacognosy, TR-06330 Ankara, Turkey
[2] Baskent Univ, Fac Pharm, Dept Pharmacognosy, TR-06810 Ankara, Turkey
[3] Gazi Univ, Fac Sci, Dept Biol, TR-06560 Ankara, Turkey
[4] Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461 USA
[5] Maharshi Dayanand Univ, Fac Pharmaceut Sci, Rohtak 124001, Haryana, India
[6] Univ Santiago de Compostela, Fac Pharm, Dept Organ Chem, Santiago De Compostela 15782, Spain
[7] Univ Cent Chile, Fac Ciencias Salud, Inst Invest & Postgrad, Santiago 8330507, Chile
关键词
Malvaceae; Malva neglecta; cytokine; endometriosis; polyphenol; quantitative real-time polymerase chain reaction; ENDOTHELIAL GROWTH-FACTOR; IN-VITRO; MEDICINAL-PLANTS; FOLK MEDICINE; TRADITIONAL MEDICINE; GENE-EXPRESSION; STROMAL CELLS; RESVERATROL; ANGIOGENESIS; ALPHA;
D O I
10.3390/molecules27227869
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Leaves and aerial parts of Malva neglecta Wallr. have been traditionally used in Anatolia for the treatment of pain, inflammation, hemorrhoids, renal stones, constipation, and infertility. This study investigated the effects of M. neglecta leaves in a rat endometriosis model. The dried plant material was extracted with n-hexane, ethyl acetate, and methanol, successively. Experimental endometriosis was surgically induced in six-week-old female, non-pregnant, Wistar albino rats by autotransplant of endometrial tissue to the abdominal wall. After twenty-eight days, rats were evaluated for a second laparotomy. Endometrial foci areas were assessed, and intraabdominal adhesions were scored. Rats were divided into five groups as control, n-hexane, ethyl acetate, methanol, and aqueous extracts, as well as reference. At the end of the treatment, all rats were sacrificed and endometriotic foci areas and intraabdominal adhesions were re-evaluated and compared with the previous findings. Moreover, peritoneal fluid was collected to detect tumor necrosis factor- alpha (TNF-alpha), vascular endothelial growth factor (VEGF), and interleukin-6 (IL-6) levels, and cDNA synthesis, and a quantitative real-time polymerase chain reaction (PCR) test was done. The phytochemical content of the most active extract was determined using High-Performance Liquid Chromatography (HPLC). Both endometrial volume and adhesion score decreased significantly in the group treated with methanol extract. In addition, significant decreases were observed in TNF-alpha, VEGF, and IL-6 levels in animals administered methanol extract. HPLC results showed that the activity caused by the methanol extract of M. neglecta was due to the polyphenols. Taken together, these novel findings indicate that M. neglecta may be a promising alternative for the treatment of endometriosis.
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页数:16
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