Isolation and characterization of 16 polymorphic microsatellite loci for Euchiloglanis kishinouyei

被引:7
作者
Li, Y. [1 ]
Wang, J. [1 ]
Zhao, K. [2 ]
Xie, B. [3 ]
Peng, Z. [1 ]
机构
[1] Southwest Univ Sch Life Sci, Minist Educ, Key Lab Freshwater Fish Reprod & Dev, Chongqing 400715, Peoples R China
[2] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China
[3] Neijiang Normal Univ, Coll Life Sci, Conservat & Utilizat Fish Resources Upper Reaches, Neijiang 641112, Peoples R China
基金
中国国家自然科学基金;
关键词
454; sequencing; genetic marker; sisorids; PHYLOGENETIC-RELATIONSHIPS; SOFTWARE; FISHES;
D O I
10.1111/jfb.12472
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A total of 16 novel polymorphic microsatellite marker loci were isolated from a genomic library of Euchiloglanis kishinouyei and further characterized using a sample from a wild population consisting of 40 individuals. The number of alleles among loci ranged from three to 17 and the observed and expected heterozygosities ranged from 0.030 to 0.950 and 0.163 to 0.980, respectively. The average polymorphic information content (PIC) of all loci was 0.450. (C) 2014 The Fisheries Society of the British Isles
引用
收藏
页码:972 / 977
页数:6
相关论文
共 27 条
[1]   Fast, cost-effective development of species-specific microsatellite markers by genomic sequencing [J].
Abdelkrim, Jawad ;
Robertson, Bruce C. ;
Stanton, Jo-Ann L. ;
Gemmell, Neil J. .
BIOTECHNIQUES, 2009, 46 (03) :185-+
[2]  
[Anonymous], 1913, FISH REC INDIAN MUSE
[3]  
CALLEN DF, 1993, AM J HUM GENET, V52, P922
[4]   Microsatellites and their genomic distribution, evolution, function and applications: A review with special reference to fish genetics [J].
Chistiakov, Dimitry A. ;
Hellemans, Bart ;
Volckaert, Filip A. M. .
AQUACULTURE, 2006, 255 (1-4) :1-29
[5]  
CHU X-L, 1981, Zoological Research, V2, P25
[6]  
Datta A. K., 1962, REC INDIAN MUS, V59, P245
[7]   Arlequin (version 3.0): An integrated software package for population genetics data analysis [J].
Excoffier, Laurent ;
Laval, Guillaume ;
Schneider, Stefan .
EVOLUTIONARY BIOINFORMATICS, 2005, 1 :47-50
[8]   MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design [J].
Faircloth, Brant C. .
MOLECULAR ECOLOGY RESOURCES, 2008, 8 (01) :92-94
[9]   Isolating microsatellite DNA loci [J].
Glenn, TC ;
Schable, NA .
MOLECULAR EVOLUTION: PRODUCING THE BIOCHEMICAL DATA, PART B, 2005, 395 :202-222
[10]  
Guo Xian-Guang, 2004, Acta Hydrobiologica Sinica, V28, P260