Determination of Flavonoids and Resveratrol in Wine by Turbulent-Flow Chromatography-LC-MS

被引：22

作者：

Presta, Michele Antoniuk ^{[1
,2
]}

Bruyneel, Ben ^{[1
]}

Zanella, Renato ^{[2
]}

Kool, Jeroen ^{[1
]}

Krabbe, Johannes G. ^{[1
]}

Lingeman, Henk ^{[1
]}

机构：

[1] Vrije Univ Amsterdam, Fac Sci, Biomol Anal Sect, NL-1081 HV Amsterdam, Netherlands

[2] Univ Fed Santa Maria, Dept Chem, BR-97105900 Santa Maria, RS, Brazil

来源：

CHROMATOGRAPHIA | 2009年 / 69卷

关键词：

Column liquid chromatography mass spectrometry; Turbulent-flow chromatography; Flavonoids; Resveratrol in wine; PERFORMANCE LIQUID-CHROMATOGRAPHY; PRESSURE CHEMICAL-IONIZATION; MASS-SPECTROMETRY; TRANS-RESVERATROL; PHENOLIC-ACIDS; CANCER; EXTRACTION; FRUITS; RISK; HPLC;

D O I：

10.1365/s10337-009-1132-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Turbulent-flow chromatography (TFC) on-line coupled to liquid chromatography mass spectrometry (LC-MS) is used to determine flavonoids and resveratrol in different types of wines. A fully automated system was developed in which 10 mL of sample (diluted wine) was passed over a TFC column, after which the retained analytes were separated by reversed-phase LC and detected by negative ion mode atmospheric-pressure chemical ionization (APCI) MS. The method proved to be fast, non-laborious, robust and sensitive. The feasibility of the method was tested on several red, white and rose wines. Quantitation of resveratrol was possible using the standard addition procedure. Red wine showed the highest amount of resveratrol (4 mg L-1), while rose and white wine contained concentrations which were about ten fold lower.

引用

页码：167 / 173

页数：7

共 28 条

[1] Therapeutic potential of resveratrol:: the in vivo evidence [J].

Baur, Joseph A. ;

Sinclair, David A. .

NATURE REVIEWS DRUG DISCOVERY, 2006, 5 (06) :493-506

[2] Resveratrol acts as a mixed agonist/antagonist for estrogen receptors α and β [J].

Bowers, JL ;

Tyulmenkov, VV ;

Jernigan, SC ;

Klinge, CM .

ENDOCRINOLOGY, 2000, 141 (10) :3657-3667

[3] Chromatographic analysis of trans resveratrol in Italian wines: Comparisons between FL, UV and MS detection [J].

Buiarelli, F. ;

Coccioli, F. ;

Jasionowska, R. ;

Merolle, M. ;

Terracciano, A. .

CHROMATOGRAPHIA, 2006, 64 (7-8) :475-481

[4]

*COMM EUR COMM, 2007, 20073131 SANCO

[5] Liquid chromatography with atmospheric pressure chemical ionization and electrospray ionization mass spectrometry of flavonoids with triple-quadrupole and ion-trap instruments [J].

de Rijke, E ;

Zappey, H ;

Ariese, F ;

Gooijer, C ;

Brinkman, UAT .

JOURNAL OF CHROMATOGRAPHY A, 2003, 984 (01) :45-58

[6] High turbulence liquid chromatography online extraction and tandem mass spectrometry for the simultaneous determination of suberoylanilide hydroxamic acid and its two metabolites in human serum [J].

Du, LH ;

Musson, DG ;

Wang, AQ .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (13) :1779-1787

[7] Determination of red wine flavonoids by HPLC and effect of aging [J].

Fang, Fang ;

Li, Jing-Ming ;

Pan, Qiu-Hong ;

Huang, Wei-Dong .

FOOD CHEMISTRY, 2007, 101 (01) :428-433

[8] Analytical procedure for the in-vial derivatization- extraction of phenolic acids and flavonoids in methanolic and aqueous plant extracts followed by gas chromatography with mass-selective detection [J].

Fiamegos, YC ;

Nanos, CG ;

Vervoort, J ;

Stalikas, CD .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1041 (1-2) :11-18

[9] Resveratrol induces apoptosis and inhibits angiogenesis in human breast cancer xenografts in vivo [J].

Garvin, S ;

Öllinger, K ;

Dabrosin, C .

CANCER LETTERS, 2006, 231 (01) :113-122

[10] Improved methods for the extraction and analysis of isoflavones from soy-containing foods and nutritional supplements by reversed-phase high-performance liquid chromatography and liquid chromatography-mass spectrometry [J].

Griffith, AP ;

Collison, MW .

JOURNAL OF CHROMATOGRAPHY A, 2001, 913 (1-2) :397-413

← 1 2 3 →