A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

被引：34

作者：

Liu, Feng ^{[1
]}

Ding, Ailing ^{[1
]}

Zheng, Jiushang ^{[1
]}

Chen, Jiucun ^{[1
]}

Wang, Bin ^{[1
]}

机构：

[1] Southwest Univ, Fac Mat & Energy, Inst Clean Energy & Adv Mat, Chongqing 400715, Peoples R China

来源：

SENSORS | 2018年 / 18卷 / 06期

基金：

中国国家自然科学基金;

关键词：

aptamer; OTA; structure switching; ZnPPIX; fluorescence; TURN-ON DETECTION; G-QUADRUPLEX; ELECTROCHEMICAL APTASENSOR; ULTRASENSITIVE DETECTION; IMPEDIMETRIC APTASENSOR; QUANTUM DOTS; DNA APTAMER; NANOPARTICLES; GOLD; AMPLIFICATION;

D O I：

10.3390/s18061769

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-protoporphyrin IX probe due to the specific bind to G-quadruplex. The simple method exhibits high sensitivity towards OTA with a detection limit of 0.03 nM and excellent selectivity over other mycotoxins. In addition, the successful detection of OTA in real samples represents a promising application in food safety.

引用

页数：10

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