The Mandibular Cartilage Metabolism is Altered by Damaged Subchondral Bone from Traumatic Impact Loading

被引：25

作者：

Lin, Yu-Yu ^{[1
]}

Tanaka, Nobuaki ^{[1
]}

Ohkuma, Satoru ^{[1
]}

Kamiya, Takashi ^{[1
]}

Kunimatsu, Ryo ^{[1
]}

Huang, Yu-Ching ^{[1
]}

Yoshioka, Motoko ^{[1
]}

Mitsuyoshi, Tomomi ^{[1
]}

Tanne, Yuki ^{[1
]}

Tanimoto, Kotaro ^{[1
]}

Tanaka, Eiji ^{[2
]}

Tanne, Kazuo ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Dept Orthodont & Craniofacial Dev Biol, Minami Ku, Hiroshima 7348553, Japan

[2] Univ Tokushima, Grad Sch Oral Sci, Dept Orthodont & Dentofacial Orthoped, Tokushima 7708504, Japan

来源：

ANNALS OF BIOMEDICAL ENGINEERING | 2009年 / 37卷 / 07期

关键词：

Impact-loading; Subchondral bone; Cartilage; Co-culture; Mandibular condyle; IDIOPATHIC CONDYLAR RESORPTION; ARTICULAR-CARTILAGE; TEMPOROMANDIBULAR DISORDERS; HUMAN CHONDROCYTES; HYALURONIC-ACID; SYNOVIAL-FLUID; TNF-ALPHA; OSTEOARTHRITIS; JOINT; INTERLEUKIN-1;

D O I：

10.1007/s10439-009-9696-z

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Osteoarthritis (OA) in the temporomandibular joint (TMJ) is a degenerative disease caused by excessive external loading. Recently, it was reported that the damage in the mineralized subchondral bone caused by traumatic impact-loading is responsible for the initiation and progression of cartilage degeneration. Thus far, we have hypothesized that cytokines released from damaged subchondral bone from impact-loading affect the cartilage catabolism under pathological conditions. An impactor of 200 gw was dropped onto the top of a porcine mandibular condyle. After organ culture for 2 days, we investigated the association between the subchondral bone and cartilage using histological and biochemical experiments. The impact-loading induced the expression of IL-1 beta immunohistochemically and prominently up-regulated IL-1 alpha and IL-1 beta mRNA levels in subchondral bone. We confirmed a significant decrease in type II collagen and aggrecan mRNA expressions in chondrocytes by co-culture with osteoblasts after impact-loading, and significant increase in mRNA and protein expressions of IL-1 beta in subchondral osteoblasts from impact-loaded subchondral bone. The mRNA expressions of type II collagen, aggrecan, and type X collagen in chondrocytes were decreased significantly by the co-culture with osteoblasts pre-treated by IL-1 beta, -6, and TNF-alpha. Among them, osteoblasts pre-treated by IL-1 beta affected chondrocytes most strongly. It was also shown that IL-1 beta-treated osteoblasts enhanced the MMP-1 mRNA level most markedly in chondrocytes among the four cytokines. These results suggest that the TMJ subjected to impact-loading can increase directly IL-1 beta synthesis in the subchondral region, subsequently altering the metabolism of adjacent cartilage and may eventually resulting in the onset and progression of TMJ-OA.

引用

页码：1358 / 1367

页数：10

共 45 条

[1] INTERLEUKIN-1 RECEPTOR ANTAGONIST [J].