Enhancing the Nucleolytic Resistance and Bioactivity of Functional Nucleic Acids by Diverse Nanostructures through in Situ Polymerization-Induced Self-assembly

被引：22

作者：

Yang, Lu ^{[1
,2
,3
]}

Liang, Mingwei ^{[4
]}

Cui, Cheng ^{[5
]}

Li, Xiaowei ^{[1
,2
,3
]}

Li, Long ^{[1
,2
,3
]}

Pan, Xiaoshu ^{[1
,2
,3
]}

Yazd, Hoda Safari ^{[1
,2
,3
]}

Hong, Min ^{[1
,2
,3
]}

Lu, Jianrong ^{[4
]}

Cao, Y. Charles ^{[1
,2
,3
]}

Tan, Weihong ^{[5
,6
]}

机构：

[1] Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA

[2] Univ Florida, Dept Physiol & Funct Genom, UF Hlth Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA

[3] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA

[4] Univ Florida, Coll Med, Dept Biochem & Mol Biol, UF Hlth Canc Ctr, Gainesville, FL 32610 USA

[5] Hunan Univ, Mol Sci & Biomed Lab, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn,Coll Life Sci,Collaborat In, Changsha 410082, Peoples R China

[6] Univ Chinese Acad Sci, Canc Hosp, Zhejiang Tumor Hosp, Inst Canc & Basic Med IBMC,Chinese Acad Sci, Hangzhou 310022, Zhejiang, Peoples R China

来源：

CHEMBIOCHEM | 2021年 / 22卷 / 04期

关键词：

aptamers; nucleic acids; nanostructures; polymerization; self-assembly; COPOLYMER NANO-OBJECTS; DNA; DELIVERY;

D O I：

10.1002/cbic.202000712

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Functional nucleic acids (FNAs) are garnering tremendous interest owing to their high modularity and unique bioactivity. Three-dimensional FNAs have been developed to overcome the issues of nuclease degradation and limited cell uptake. We have developed a new facile approach to the synthesis of multiple three-dimensional FNA nanostructures by harnessing photo-polymerization-induced self-assembly. Sgc8 aptamer and CpG oligonucleotide were modified as macro chain-transfer reagents to mediate in situ polymerization and self-assembly. Diverse structures, including micelles, rods, and short worms, afford these two FNAs afford these two FNAs with higher nuclease resistance in serum serum, greater cellular uptake efficiency, and increased bioactivity.

引用

页码：754 / 759

页数：6

共 36 条

[1] Drug-Loaded Polymeric Spherical Nucleic Acids: Enhancing Colloidal Stability and Cellular Uptake of Polymeric Nanoparticles through DNA Surface-Functionalization [J].

Banga, Resham J. ;

Krovi, Sal Archana ;

Narayan, Suguna P. ;

Sprangers, Anthony J. ;

Liu, Guoliang ;

Mirkin, Chad A. ;

Nguyen, SonBinh T. .

BIOMACROMOLECULES, 2017, 18 (02) :483-489

[2] Polymer bioconjugates: Modern design concepts toward precision hybrid materials [J].

Chen, Chaojian ;

Ng, David Yuen Wah ;

Weil, Tanja .

PROGRESS IN POLYMER SCIENCE, 2020, 105

[3] Mouse SIRT3 Attenuates Hypertrophy-Related Lipid Accumulation in the Heart through the Deacetylation of LCAD [J].

Chen, Tongshuai ;

Liu, Junni ;

Li, Na ;

Wang, Shujian ;

Liu, Hui ;

Li, Jingyuan ;

Zhang, Yun ;

Bu, Peili .

PLOS ONE, 2015, 10 (03)

[4] A pH-Triggered, Fast-Responding DNA Hydrogel [J].

Cheng, Enjun ;

Xing, Yongzheng ;

Chen, Ping ;

Yang, Yang ;

Sun, Yawei ;

Zhou, Dejian ;

Xu, Lijin ;

Fan, Qinghua ;

Liu, Dongsheng .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (41) :7660-7663

[5] Polymerization-Induced Coassembly of Enzyme-Polymer Conjugates into Comicelles with Tunable and Enhanced Cascade Activity [J].

Chiang, Chi-Wei ;

Liu, Xinyu ;

Sun, Jiawei ;

Guo, Jianwen ;

Tao, Lei ;

Gao, Weiping .

NANO LETTERS, 2020, 20 (02) :1383-1387

[6] Mechanism for the endocytosis of spherical nucleic acid nanoparticle conjugates [J].

Choi, Chung Hang J. ;

Hao, Liangliang ;

Narayan, Suguna P. ;

Auyeung, Evelyn ;

Mirkin, Chad A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (19) :7625-7630

[7] Spherical Nucleic Acids [J].

Cutler, Joshua I. ;

Auyeung, Evelyn ;

Mirkin, Chad A. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (03) :1376-1391

[8] Dispersity effects in polymer self-assemblies: a matter of hierarchical control [J].

Doncom, Kay E. B. ;

Blackman, Lewis D. ;

Wright, Daniel B. ;

Gibson, Matthew I. ;

O'Reilly, Rachel K. .

CHEMICAL SOCIETY REVIEWS, 2017, 46 (14) :4119-4134

[9] Shape effects of filaments versus spherical particles in flow and drug delivery [J].

Geng, Yan ;

Dalhaimer, Paul ;

Cai, Shenshen ;

Tsai, Richard ;

Tewari, Manorama ;

Minko, Tamara ;

Discher, Dennis E. .

NATURE NANOTECHNOLOGY, 2007, 2 (04) :249-255

[10]

Häcker G, 2002, IMMUNOLOGY, V105, P245

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