Age-related changes in angiogenesis in human dermis

被引：50

作者：

Gunin, Andrei G. ^{[1
]}

Petrov, Vadim V. ^{[1
]}

Golubtzova, Natalia N. ^{[1
]}

Vasilieva, Olga V. ^{[1
]}

Kornilova, Natalia K. ^{[1
]}

机构：

[1] Chuvash State Univ, Sch Med, Dept Obstet & Gynecol, Cheboksary 428015, Russia

来源：

EXPERIMENTAL GERONTOLOGY | 2014年 / 55卷

基金：

俄罗斯基础研究基金会;

关键词：

Skin; Aging; Angiogenesis; Blood vessels; Von Willebrand factor; CD31; VEGF; Dll4; Jag-1; ESTRADIOL-INDUCED PROLIFERATION; ENDOTHELIAL GROWTH-FACTOR; VON-WILLEBRAND-FACTOR; HUMAN-SKIN; HYPERPLASIA FORMATION; MOLECULAR-MECHANISMS; TRANSGENIC MICE; MOUSE UTERUS; CELLS; OLD;

D O I：

10.1016/j.exger.2014.04.010

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Present research is aimed to examine the number of dermal blood vessels, vascular endothelial growth factor (VEGF), delta-like ligand 4(Dll4) and Jagged-1 (Jag-1) in dermal blood vessels of human from 20 weeks of pregnancy to 85 years old. Numbers and proliferative activity of dermal fibroblast-like cells were also examined. Blood vessels were viewed with immunohistochemical staining for von Willebrand factor or CD31. VEGF, Dll4, Jag-1, and proliferating cell nuclear antigen (PCNA) were detected immunohistochemically. Results showed that the numbers of fibroblast-like cells, PCNA positive fibroblast-like cells, von Willebrand factor positive or CD31 positive blood vessels in dermis are dramatically decreased with age. The intensity of immunohistochemical staining for VEGF or Jag-1 in blood vessels of dermis is increased from antenatal to deep old period. The degree of immunohistochemical staining of dermal blood vessels for Dll4 has gone up from 20-40 weeks of pregnancy to early life period (0-20 years), and further decreased below antenatal values. Age-related decrease in the number of dermal blood vessels is suggested to be due to an impairment of VEGF signaling and to be mediated by Dll4 and Jag-1. It may be supposed that diminishing in blood supply of dermis occurring with age is a cause of a decrease in the number and proliferative pool of dermal fibroblasts. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：143 / 151

页数：9