Bioactive DOPA melanin isolated and characterised from a marine actinobacterium Streptomyces sp MVCS6 from Versova coast

被引：22

作者：

Sivaperumal, P. ^{[1
,2
]}

Kamala, K. ^{[1
]}

Rajaram, R. ^{[2
]}

机构：

[1] ICAR Deemed Univ, Cent Inst Fisheries Educ, Bombay 400061, Maharashtra, India

[2] Bharathidasan Univ, Dept Marine Sci, Tiruchirappalli, Tamil Nadu, India

来源：

NATURAL PRODUCT RESEARCH | 2015年 / 29卷 / 22期

关键词：

melanin; FT-IR; antibacterial; antioxidant; cytotoxicity activity; PIGMENT; BIOSYNTHESIS;

D O I：

10.1080/14786419.2014.988712

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The melanin pigment produced from Streptomyces sp., MVCS6 was isolated and dihydroxyphenyalanine (DOPA) melanin compound was biochemically identified and spectroscopically characterised (ultraviolet and FT-IR). DOPA melanin showed a promising activity as an antibacterial natural product against 12 pathogenic bacteria from hospital isolations, particularly, against Pseudomonas aeruginosa RMMH7 (inhibition zone of 18 +/- 0.02 at 30g/disc, and MIC of 10 +/- 0.02g/mL) and Vibrio parahaemolytics RMMH12 (inhibition zone of 15mm +/- 0.03 at 30g/disc, and MIC of 14 +/- 0.02g/mL). Moreover, in vitro evaluation of reducing power (Ascorbic Acid Equivalent (160g/mL)), DPPH radical-scavenging (89%), NO-scavenging (72%) and lipid peroxidation activities (89.6%) were determined. Cytotoxicity of DOPA melanin against cervical cancer cell line showed a dose-response activity, and IC50 value was found to be 300g/mL. These results would open the way to propose Streptomyces sp. MVCS6 as a promising source of bioactive eumelanin with therapeutic potential in medicine.

引用

页码：2117 / 2121

页数：5