Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration

被引：18

作者：

Cao, Zigang ^{[1
]}

Meng, Yunlong ^{[2
]}

Gong, Fanghua ^{[3
]}

Xu, Zhaopeng ^{[2
]}

Liu, Fasheng ^{[1
]}

Fang, Mengjie ^{[1
]}

Zou, Lufang ^{[1
]}

Liao, Xinjun ^{[1
]}

Wang, Xinjuan ^{[4
]}

Luo, Lingfei ^{[4
]}

Li, Xiaokun ^{[3
]}

Lu, Huiqiang ^{[1
,2
]}

机构：

[1] Jinggangshan Univ, Jiangxi Key Lab Dev Biol Organs, Coll Life Sci,Affiliated Hosp, Jiangxi Engn Lab Zebrafish Modeling & Drug Screen, Jian 343009, Jiangxi, Peoples R China

[2] Gannan Normal Univ, Ctr Drug Screening & Res, Sch Geog & Environm Engn, Ganzhou 341000, Jiangxi, Peoples R China

[3] Wenzhou Med Univ, Sch Pharm, Wenzhou 325000, Zhejiang, Peoples R China

[4] Southwest Univ, Inst Dev Biol & Regenerat Med, Chongqing 400715, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2021年 / 118卷 / 02期

基金：

国家重点研发计划;

关键词：

calcineurin; polarity; fin regeneration; zebrafish; TAIL FIN; PLANARIAN REGENERATION; ISOMETRIC GROWTH; PROLIFERATION; MACROPHAGES; INHIBITION; COMPLEXES; DEFINES; CELLS; FK506;

D O I：

10.1073/pnas.2009539118

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Planarian flatworms regenerate their heads and tails from anterior or posterior wounds and this regenerative blastema polarity is controlled by Wnt/beta-catenin signaling. It is well known that a regeneration blastema of appendages of vertebrates such as fish and amphibians grows distally. However, it remains unclear whether a regeneration blastema in vertebrate appendages can grow proximally. Here, we show that a regeneration blastema in zebrafish fins can grow proximally along the proximodistal axis by calcineurin inhibition. We used fin excavation in adult zebrafish to observe unidirectional regeneration from the anterior cut edge (ACE) to the posterior cut edge (PCE) of the cavity and this unidirectional regeneration polarity occurs as the PCE fails to build blastemas. Furthermore, we found that calcineurin activities in the ACE were greater than in the PCE. Calcineurin inhibition induced PCE blastemas, and calcineurin hyperactivation suppressed fin regeneration. Collectively, these findings identify calcineurin as a molecular switch to specify the PCE blastema of the proximodistal axis and regeneration polarity in zebrafish fin.

引用

页数：8

共 40 条

[21] EpCAM Is an Endoderm-Specific Wnt Derepressor that Licenses Hepatic Development [J].