Cellular in vivo 3D imaging of the cornea by confocal laser scanning microscopy

被引：40

作者：

Bohn, Sebastian ^{[1
]}

Sperlich, Karsten ^{[1
]}

Allgeier, Stephan ^{[2
]}

Bartschat, Andreas ^{[2
]}

Prakasam, Ruby ^{[1
]}

Reichert, Klaus-Martin ^{[2
]}

Stolz, Heinrich ^{[3
]}

Guthoff, Rudolf ^{[1
]}

Mikut, Ralf ^{[2
]}

Koehler, Bernd ^{[2
]}

Stachs, Oliver ^{[1
]}

机构：

[1] Univ Med Ctr Rostock, Dept Ophthalmol, D-18057 Rostock, Germany

[2] Karlsruhe Inst Technol, Inst Automat & Appl Informat, D-76131 Karlsruhe, Germany

[3] Univ Rostock, Inst Phys, D-18059 Rostock, Germany

来源：

BIOMEDICAL OPTICS EXPRESS | 2018年 / 9卷 / 06期

关键词：

SUBBASAL NERVE PLEXUS; GUIDED EYE-MOVEMENTS; THICKNESS; HRT;

D O I：

10.1364/BOE.9.002511

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present an in vivo confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286 x 396 mu m(3). The microscope objective used is equipped with a piezo actuator for automated, fast and precise closed-loop focal plane control. Furthermore, we present a novel concave surface contact cap, which significantly reduces eye movements by up to 87%, hence increasing the overlapping image area of the whole stack. This increases the cuboid volume of the generated 3D reconstruction significantly. The possibility to generate oblique sections using isotropic volume stacks opens the window to slit lamp microscopy on a cellular level. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：2511 / 2525

页数：15

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