Cellular in vivo 3D imaging of the cornea by confocal laser scanning microscopy

被引：40

作者：

Bohn, Sebastian ^{[1
]}

Sperlich, Karsten ^{[1
]}

Allgeier, Stephan ^{[2
]}

Bartschat, Andreas ^{[2
]}

Prakasam, Ruby ^{[1
]}

Reichert, Klaus-Martin ^{[2
]}

Stolz, Heinrich ^{[3
]}

Guthoff, Rudolf ^{[1
]}

Mikut, Ralf ^{[2
]}

Koehler, Bernd ^{[2
]}

Stachs, Oliver ^{[1
]}

机构：

[1] Univ Med Ctr Rostock, Dept Ophthalmol, D-18057 Rostock, Germany

[2] Karlsruhe Inst Technol, Inst Automat & Appl Informat, D-76131 Karlsruhe, Germany

[3] Univ Rostock, Inst Phys, D-18059 Rostock, Germany

来源：

BIOMEDICAL OPTICS EXPRESS | 2018年 / 9卷 / 06期

关键词：

SUBBASAL NERVE PLEXUS; GUIDED EYE-MOVEMENTS; THICKNESS; HRT;

D O I：

10.1364/BOE.9.002511

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present an in vivo confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286 x 396 mu m(3). The microscope objective used is equipped with a piezo actuator for automated, fast and precise closed-loop focal plane control. Furthermore, we present a novel concave surface contact cap, which significantly reduces eye movements by up to 87%, hence increasing the overlapping image area of the whole stack. This increases the cuboid volume of the generated 3D reconstruction significantly. The possibility to generate oblique sections using isotropic volume stacks opens the window to slit lamp microscopy on a cellular level. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：2511 / 2525

页数：15

共 50 条

[1] In vivo confocal laser scanning microscopy of the cornea in dry eye
Béla Erdélyi
Robert Kraak
Andrey Zhivov
Rudolf Guthoff
János Németh
Graefe's Archive for Clinical and Experimental Ophthalmology, 2007, 245 : 39 - 44
[2] In vivo confocal laser scanning microscopy of the cornea in dry eye
Erdelyi, Bela
Kraak, Robert
Zhivov, Andrey
Guthoff, Rudolf
Nemeth, Janos
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 2007, 245 (01) : 39 - 44
[3] In vivo confocal laser scanning microscopy of the cornea comparable to slit lamp section imaging
Guthoff, RF
Stave, J
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2005, 46
[4] ADVANTAGES OF 3D LASER SCANNING CONFOCAL MICROSCOPY
Shingledecker, John
Siefert, John
Purdy, Daniel
Tedesco, Jonathan
Szafarczyk, Andrew
ADVANCED MATERIALS & PROCESSES, 2016, 174 (10): : 22 - 25
[5] Examinations of innervation of the human cornea by confocal in vivo microscopy and confocal laser scanning fluorescence microscopy
Richter, A
Hahnel, C
Somodi, S
Slowik, C
Weiss, DG
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1997, 38 (04) : 1870 - 1870
[6] Confocal in vivo microscopy and confocal laser scanning fluorescence microscopy of the human cornea in keratoconus and bullous keratopathy
Somodi, S
Hahnel, C
Slowik, C
Richter, A
Guthoff, R
Weiss, DG
SOE '97 - XI CONGRESS OF THE EUROPEAN SOCIETY OF OPHTHALMOLOGY, VOLS 1 AND 2, 1997, : 157 - 164
[7] Multiwavelength confocal laser scanning microscopy of the cornea
Bohn, Sebastian
Sperlich, Karsten
Stahnke, Thomas
Schuenemann, Melanie
Stolz, Heinrich
Guthoff, Rudolf F.
Stachs, Oliver
BIOMEDICAL OPTICS EXPRESS, 2020, 11 (10) : 5689 - 5700
[8] SCANNING SLIT CONFOCAL MICROSCOPY OF THE IN-VIVO CORNEA
MASTERS, BR
OPTICAL ENGINEERING, 1995, 34 (03) : 684 - 692
[9] In vivo imaging of intradermal tattoos by confocal scanning laser microscopy
O'goshi, K
Suihko, C
Serup, J
SKIN RESEARCH AND TECHNOLOGY, 2006, 12 (02) : 94 - 98
[10] In Vivo Imaging of Lichen Nitidus With Confocal Laser Scanning Microscopy
Liu, Huaxu
Chen, Shengli
Shi, Zhongxiang
Zhang, Furen
ARCHIVES OF DERMATOLOGY, 2011, 147 (01) : 142 - 142

← 1 2 3 4 5 →