Monitoring of extracellular pyruvate, lactate, and ascorbic acid during cerebral ischemia: a microdialysis study in awake gerbils

被引：12
作者：
Cheng, FC
Yang, LL
Yang, DY [1 ]
Tsai, TH
Lee, CW
Chen, SH
机构：
[1] Taichung Vet Gen Hosp, Dept Emergency, Taichung 407, Taiwan
[2] Taichung Vet Gen Hosp, Dept Med Res, Taichung 407, Taiwan
[3] Natl Res Inst Chinese Med, Dept Pharmacol, Taipei 112, Taiwan
[4] Natl Chung Hsing Univ, Dept Chem, Taichung 402, Taiwan
来源：
JOURNAL OF CHROMATOGRAPHY A | 2000年 / 870卷 / 1-2期
关键词：
microdialysis; gerbils; pyruvate; lactate; ascorbic acid;
D O I：
10.1016/S0021-9673(99)00905-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In vivo microdialysis coupled with liquid chromatography was developed for the continuous monitoring of brain neurochemicals during cerebral ischemia in awake, free moving gerbils. The dead volume of the microdialysis system was estimated to be less than 30 mu l. The detection Limits of the present assay were 0.2 to 2.0 mu M for analytes at a signal to noise ratio of five. To validate this assay, a focal cerebral ischemia was produced by occlusion of one common carotid artery for 60 min and then reperfusion for additional 3 h in awake gerbils. A microdialysis probe was inserted into the striatum of the gerbil, Dialysates were autoinjected and analyzed extracellular pyruvate, lactate, and ascorbic acid by liquid chromatography with a UV detector during cerebral ischemia. Significant changes of pyruvate and the lactate/pyruvate ratio were observed. Biphasic and dynamic changes in ascorbic acid and lactate were proposed to correlate a secondary damage. This assay can be used as a tool to study dynamic changes of brain neurochemicals in awake animals. (C) 2000 Elsevier Science BN. All rights reserved.
引用
收藏
页码：389 / 394
页数：6
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