Singlet order conversion and parahydrogen-induced hyperpolarization of 13C nuclei in near-equivalent spin systems

被引：48

作者：

Eills, James ^{[1
]}

Stevanato, Gabriele ^{[1
]}

Bengs, Christian ^{[2
]}

Gloggler, Stefan ^{[1
]}

Elliott, Stuart J. ^{[1
]}

Alonso-Valdesueiro, Javier ^{[1
]}

Pileio, Giuseppe ^{[1
]}

Levitt, Malcolm H. ^{[1
]}

机构：

[1] Univ Southampton, Southampton SO17 1BJ, Hants, England

[2] Goethe Univ, D-60323 Frankfurt, Germany

来源：

JOURNAL OF MAGNETIC RESONANCE | 2017年 / 274卷

基金：

英国工程与自然科学研究理事会;

关键词：

PHIP; Singlet states; Hyperpolarization; Parahydrogen; SLIC; PARA-HYDROGEN; INDUCED POLARIZATION; STORAGE; STATES;

D O I：

10.1016/j.jmr.2016.11.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have demonstrated two radiofrequency pulse methods which convert the nuclear singlet order of proton spin pairs into the magnetisation of nearby C-13 nuclei. These irradiation schemes work well in the near-equivalence regime of the three-spin system, which applies when the difference in the two H-1-C-13 couplings is much smaller than the H-1-H-1 coupling. We use pulse sequences to generate thermally polarized singlet states in a reproducible manner, and study the singlet-to-magnetisation transfer step. Preliminary results demonstrate a parahydrogen-enhanced C-13 polarization level of at least 9%, providing a signal enhancement factor of more than 9000, using 50% enriched parahydrogen. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：163 / 172

页数：10

共 42 条

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