Transcription factors regulating uspA genes in Catharanthus roseus

被引:2
|
作者
Bahieldin, Ahmed [1 ,2 ]
Atef, Ahmed [1 ]
Shokry, Ahmed M. [1 ,3 ]
Al-Karim, Saleh [1 ]
Al Attas, Sanaa G. [1 ]
Gadallah, Nour O. [1 ,4 ]
Edris, Sherif [1 ,2 ,5 ]
Al-Kordy, Magdy A. [1 ,4 ]
Hassan, Sabah M. [1 ,2 ]
Abo-Aba, Salah [1 ,6 ]
El-Domyati, Fotouh M. [1 ,2 ]
机构
[1] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, POB 80141, Jeddah 21589, Saudi Arabia
[2] Ain Shams Univ, Dept Genet, Fac Agr, Cairo, Egypt
[3] ARC, AGERI, Giza, Egypt
[4] Natl Res Ctr, Genet Engn & Biotechnol Div, Genet & Cytol Dept, Dokki, Egypt
[5] King Abdulaziz Univ, Fac Med, Princess Al Jawhara Al Brahim Ctr Excellence Res, Jeddah, Saudi Arabia
[6] Natl Res Ctr, Microbial Genet Dept, Genet Engn & Biotechnol Div, Giza, Egypt
关键词
Transcription factor; Co-expression; Erf; BHLH; WRKY; RNA-Seq; SqRT-PCR; GENOME-WIDE; ARABIDOPSIS; ETHYLENE; EXPRESSION; TOMATO; FAMILY; BINDING; ACID; BIOSYNTHESIS; RESISTANCE;
D O I
10.1016/j.crvi.2016.10.004
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
RNA-Seq of the Catharanthus roseus SRA database was done in order to detect putative universal stress proteins (USPs) and their possible controlling factors. Previous analysis indicated the existence and characterization of uspA-like genes. In silico analysis of RNA-Seq database in several plant tissues revealed the possible functions and regulations of some uspA-like transcripts whose transcription factors (TFs) that might drive their expression were detected. BLAST indicated the existence of TF superfamilies erf (ethyleneresponsive TF), bHLH (basic helix-loop-helix) and WRKY that might regulate several uspA-like genes. This data was proven via semi-quantitative RT-PCR in four plant tissues. Several of these transcription factor superfamilies are known for their action in the plant defense against biotic and abiotic stresses. (C) 2016 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:1 / 6
页数:6
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