A novel water-soluble β-(1→6)-D-glucan isolated from the fruit bodies of Bulgaria inquinans (Fries)

被引：23

作者：

Bi, Hongtao ^{[1
]}

Ni, Xiuzhen ^{[1
,2
]}

Liu, Xiaoyu ^{[1
,3
]}

Iteku, Jeff ^{[1
]}

Tai, Guihua ^{[1
]}

Zhou, Yifa ^{[1
]}

Zhao, Jimin ^{[2
]}

机构：

[1] NE Normal Univ, Sch Life Sci, Changchun 130024, Peoples R China

[2] Changchun Normal Univ, Sch Life Sci, Changchun 130022, Peoples R China

[3] Shenyang Agr Univ, Coll Sci, Shenyang 110161, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2009年 / 344卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Polysaccharide; beta-(1 -> 6)-D-Glucan; Bulgaria inquinans (Fries); Methylation; BETA-D-GLUCAN; ANTITUMOR-ACTIVITY; STRUCTURAL-CHARACTERIZATION; CELL-WALL; POLYSACCHARIDES; COMPONENTS; FUNGI; CHROMATOGRAPHY; CONSTITUENTS; EXTRACT;

D O I：

10.1016/j.carres.2009.04.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A low molecular-weight polysaccharide named BiWP2 was purified from the fruit bodies of Bulgaria Inquinans (Fries) via hot-water extraction, followed by freeze-thawing and gel filtration chromatography on Sephadex G-75. Monosaccharide composition analysis revealed that BIWP2 contained exclusively glucose. High performance size exclusion chromatography (HPSEC) showed that it was a homogeneous polysaccharide fraction. Its molecular weight was estimated to be 2.6 KD and the polydispersity index (M-w/M-n) Was calculated to be 1.4. Periodate oxidation, methylation, and NMR analyses indicated that BIWP2 was a linear beta-(1 -> 6)-D-glucan without side chains. This is the first time to report a linear beta-(1 -> 6)-D-glucan with low molecular weight in non-lichenized ascomycete. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1254 / 1258

页数：5

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