Molecular and phylogenetic characterization of Theileria spp. parasites in autochthonous bovines (Mirandesa breed) in Portugal

被引：23
作者：
Brígido, C
da Fonseca, IP
Parreira, R
Fazendeiro, I
do Rosário, VE
Centeno-Lima, S
机构：
[1] Univ Nova Lisboa, Inst Higiene & Med Trop, Ctr Malaria & Outras Doencas Trop, P-1349008 Lisbon, Portugal
[2] Polo Univ Alto Ajuda, CIISA, Fac Med Vet, P-1300477 Lisbon, Portugal
[3] Univ Nova Lisboa, Inst Higiene & Med Trop, Unidade Virol UPMM, P-1349008 Lisbon, Portugal
来源：
VETERINARY PARASITOLOGY | 2004年 / 123卷 / 1-2期
关键词：
Theileria; bovine Mirandesa breed; RLB; phylogenetic analysis;
D O I：
10.1016/j.vetpar.2004.05.024
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A survey was conducted during the months of April-June 2003 in the northeast Portugal (Braganca district) in order to characterize the hemoparasite population of an autochthonous Mirandesa breed of Bos taurus. The polymerase chain reaction (PCR) analysis of the bovine blood revealed that 3 out of 116 animals were infected with Theileria and/or Babesia parasites, while reverse line blot hybridisation (RLB) analysis showed that these animals were infected with Theileria buffeli/orientalis. Cloning and sequencing confirmed the RLB results. Database sequence searches combined with phylogenetic analysis of the partial 18S ribosomal RNA gene sequences obtained enabled us to place the parasites in question as members of the T buffeli/orientalis group, confirming the PCR/RLB diagnosis. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：17 / 23
页数：7
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