Paeonol alleviated acute alcohol-induced liver injury via SIRT1/Nrf2/NF-κB signaling pathway

被引：55
作者：
Sun, Xing [1 ,2 ]
Wang, Peng [1 ,2 ]
Yao, Li-Ping [1 ,2 ]
Wang, Wei [1 ,2 ]
Gao, Yi-Meng [1 ,2 ]
Zhang, Jing [1 ,2 ]
Fu, Yu-Jie [1 ,2 ,3 ]
机构：
[1] Minist Educ, Key Lab Forest Plant Ecol, Harbin 150040, Heilongjiang, Peoples R China
[2] Northeast Forestry Univ, Engn Res Ctr Forest Biopreparat, Minist Educ, Harbin 150040, Heilongjiang, Peoples R China
[3] Beijing Forestry Univ, Coll Forestry, Beijing 100083, Peoples R China
来源：
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2018年 / 60卷
基金：
中国国家自然科学基金; 国家重点研发计划;
关键词：
Paeonol; Alcoholic liver disease; Oxidative stress; Inflammation; SIRT1; NF-KAPPA-B; INDUCED OXIDATIVE STRESS; TNF-ALPHA PRODUCTION; KUPFFER CELLS; IN-VITRO; MICE; DISEASE; EXPRESSION; PROTECTS; DAMAGE;
D O I：
10.1016/j.etap.2018.04.016
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
In this study, the beneficial effect of paeonol on acute alcohol-induced liver injury and the basic mechanisms were investigated. in vitro, HepG2 cells were treated with paeonol for 24 h before it were exposed to alcohol for 24 h. in vivo, male C57BL/6 mice were used to establish alcohol-induced liver injury models by oral gavage of alcohol (5 g/kg BW). Paeonol pretreatment showed statistically significant reduction in alcohol-induced ROS, MDA, IL-1 beta, IL-6, TNF-alpha, and nitric oxide, while GSH content was retained (P < 0.05). Furthermore, paeonol treatment resulted in the increase of Nrf2 nuclear translocation, the increase of NQO-1 and HO-1 expression, and the suppression of NF-kappa B p65 nuclear translocation. However, pretreatment with NAM (inhibitor of SIRT1) not only inhibited the effect of paeonol on reducing nuclear translocation of NF-kappa Bp65, but also inhibited the effect of paeonol on promoting the expression of nuclear Nrf2, NQO1 and HO-1. Besides, paeonol pretreatment at test doses significantly ameliorated alcohol-induced edema, hepatocyte necrosis and hepatic cord irregular. These results demonstrated that paeonol has the high potential for relieving acute alcohol-induced liver injury.
引用
收藏
页码：110 / 117
页数：8
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