Secretion of Adipocytes and Macrophages under Conditions of Inflammation and/or Insulin Resistance and Effect of Adipocytes on Preadipocytes under These Conditions

被引:8
作者
Wei, Yu Tao [1 ,2 ,3 ]
Xia, Dong Sheng [3 ,4 ]
Yang, Wen Kai [3 ,5 ]
Wang, Xian Guo [3 ]
Chen, Xin Zhong [3 ]
Dong, Nian Guo [3 ]
机构
[1] Shihezi Univ, Hosp Xingjian Prod & Construct Corps, Dept Thorac & Cardiovasc Surg, Coll Med,Hosp 2, Urumqi 830002, Xinjiang, Peoples R China
[2] Shihezi Univ, Coll Med, Hosp 1, Dept Thorac & Cardiovasc Surg, Shihezi 832008, Xinjiang, Peoples R China
[3] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Cardiovasc Surg, Wuhan 430022, Hubei, Peoples R China
[4] Henan Prov Peoples Hosp, Dept Cardiovasc Surg, Zhengzhou 450000, Henan, Peoples R China
[5] Jiangxi Prov Peoples Hosp, Dept Cardiac Surg, Nanchang 330006, Jiangxi, Peoples R China
关键词
insulin resistance; adipocytes; preadipocytes; adipokines; HUMAN ADIPOSE-TISSUE; ATHEROSCLEROSIS; ENDOCRINE; DIFFERENTIATION; ADIPOGENESIS; INFILTRATION; INDUCTION; CYTOKINES; OBESITY; PREF-1;
D O I
10.1134/S0006297914070086
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The purpose of the present study was to examine changes in preadipocytes following the coculture of preadipocytes and adipocytes and the effects on the secretion of adipocytes and macrophages following induction of inflammation and insulin resistance. Mature adipocytes and RAW264.7 macrophages were treated with lipopolysaccharide and insulin to establish models of inflammation and insulin resistance, respectively. The mRNA expression levels of IL-6, MCP-1, and TNF-alpha in all adipocyte treatment groups were significantly greater compared with the control, and that of adiponectin was less (P < 0.05). In the RAW264.7 macrophages, the mRNA expression levels of IL-6 and TNF-alpha were greater than those in the control group (P < 0.05). Moreover, the results of this study confirmed that adipocytes and macrophages increased the secretion of inflammatory factors under conditions of induced inflammation and insulin resistance. In addition, 3T3-L1 adipocytes inhibited the proliferation and differentiation of preadipocytes when cocultured with adipocytes under conditions of inflammation and/or insulin resistance, and the phenotype of preadipocytes did not change.
引用
收藏
页码:663 / 671
页数:9
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