Isolation, identification and characterization of pancreatic lipase inhibitors from Trigonella foenum-graecum seeds

被引：19

作者：

Fernando, W. I. T. ^{[1
]}

Attanayake, A. M. K. C. ^{[1
]}

Perera, H. K., I ^{[1
]}

Sivakanesan, R. ^{[1
]}

Jayasinghe, L. ^{[2
]}

Araya, H. ^{[3
]}

Fujimoto, Y. ^{[2
,3
]}

机构：

[1] Univ Peradeniya, Fac Med, Dept Biochem, Peradeniya, Sri Lanka

[2] Natl Inst Fundamental Studies, Hantana Rd, Kandy, Sri Lanka

[3] Meiji Univ, Sch Agr, Kanagawa, Kanagawa 2148571, Japan

来源：

SOUTH AFRICAN JOURNAL OF BOTANY | 2019年 / 121卷

关键词：

Lipase inhibitor; Trigonella foenum-graecum; Flavone C-glycoside; Vicenin-1; Isoschaftoside; Schaftoside; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLAVONE C-GLYCOSIDES; FENUGREEK; GLYCOSYLFLAVONES; CONSTITUENTS; EXTRACTS;

D O I：

10.1016/j.sajb.2018.10.023

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Activity-guided fractionation of the methanol extract of Trigonella foenum-graecum seeds furnished three flavone C-glycosides having pancreatic lipase inhibitory activity. Structures of these compounds were established as vicenin-1 (1), isoschaftoside (2) and schaftoside (3) by detail analysis of H-1, C-13 NMR and MS data. These compounds showed percentage inhibition 60.3% (1), 33.8% (2) and 95.5% (3) at the concentration of 250 mu g/ml and IC50 values of the inhibition were 207 mu g/ml (1), 330 mu g/ml (2) and 130 mu g/ml (3). This is the first report of the isolation of lipase inhibitors from T. foenum-graecum seeds. Temperature dependency of H-1 NMR of 3 was studied in detail. (C) 2018 SAAB. Published by Elsevier B.V. All rights reserved.

引用

页码：418 / 421

页数：4

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