Western blot analysis of the humoral response of dogs experimentally infected with Angiostrongylus vasorum (Baillet, 1866)

被引：28

作者：

Cury, MC

Guimaraes, MP

Lima, WS

Vitor, RWA

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Uberlandia, Parasitol Lab, BR-38400 Uberlandia, MG, Brazil

来源：

VETERINARY PARASITOLOGY | 2002年 / 106卷 / 01期

关键词：

Angiostrongylus vasorum; dog; Western blot analysis;

D O I：

10.1016/S0304-4017(02)00031-6

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Seven cross-bred dogs were inoculated with Angiostrongylus vasorum and serum samples were analyzed using the enzyme-linked immunosorbent assay (ELISA) and Western blot (WB). ELISA detected specific antibodies anti-A. vasorum, from 14 to 28 days after inoculation (DAI) and persisted throughout the experiment. Using WB, the main antigens detected had molecular weight of approximately 115, 102, 86, 76, 69, 56, 41, 32, 28, 20-22 and 10 kDa. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：83 / 87

页数：5

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