C-Glucosylflavonoid biosynthesis from 2-hydroxynaringenin by Desmodium uncinatum (Jacq.) (Fabaceae)

被引：18

作者：

Hamilton, Mary L. ^{[1
]}

Caulfield, John C. ^{[1
]}

Pickett, John A. ^{[1
]}

Hooper, Antony M. ^{[1
]}

机构：

[1] Rothamsted Res, Dept Biol Chem, Ctr Sustainable Pest & Dis Management, Harpenden AL5 2JQ, Herts, England

来源：

TETRAHEDRON LETTERS | 2009年 / 50卷 / 40期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Desmodium uncinatum; C-Glycosyltransferase; Biosynthesis; C-Glycosylflavonoid; 2-Hydroxynaringenin; Vitexin; Isovitexin; Deuterium labelling; STRIGA-HERMONTHICA; SPP; GLYCOSYLATION; FLAVONOIDS; INTERCROPS; CEREALS; MAIZE; WEEDS;

D O I：

10.1016/j.tetlet.2009.07.118

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[2',3',5',6'-H-2(4)]-2-Hydroxynaringenin is synthesised and incubated with commercially available UDP-glucose and the crude protein extract from Desmoduim uncinatum leaves. The organic extract produces isotopically labelled [2',3',5',6'-H-2(4)]-vitexin and [2',3',5',6'-H-2(4)]-isovitexin. Repeating the experiment with denatured protein or replacing the 2-hydroxynaringenin with [2',3',5',6'-H-2(4)]-apigenin or [2',3',5',6'-H-2(4)]- naringenin results in no observable incorporation. 2-Hydroxynaringenin is therefore the substrate for C-glucosylflavonoid biosynthesis in D. uncinatum. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：5656 / 5659

页数：4

共 18 条

[1] O-glucosyltransferase activities toward phenolic natural products and xenobiotics in wheat and herbicide-resistant and herbicide-susceptible black-grass (Alopecurus myosuroides) [J].