Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue

被引:69
作者
Lin, Ying-Hung
Lin, Yung-Ming
Teng, Yen-Ni
Hsieh, Tsui-Yu Tracy
Lin, Yi-Shing
Kuo, Pao-Lin
机构
[1] Natl Cheng Kung Univ, Coll Med, Grad Inst Basic Med Sci, Tainan 70101, Taiwan
[2] Natl Cheng Kung Univ, Coll Med, Dept Urol, Tainan 70101, Taiwan
[3] Chia Nan Univ Pharm & Sci, Dept Early Childhood Educ & Nursery, Tainan, Taiwan
[4] Welgene Biotech Co Ltd, Taipei, Taiwan
[5] Natl Cheng Kung Univ, Coll Med, Dept Obstet & Gynecol, Tainan 70101, Taiwan
关键词
microarray; testis; spermatogenesis;
D O I
10.1016/j.fertnstert.2006.04.039
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To identify novel genes that are down-regulated in the testicular tissue of infertile men. Design: Prospective study. Setting: University-based reproductive clinics and genetic laboratory. Patients: Nine patients with normal spermatogenesis, and 15 patients with maturation arrest (MA) or Sertoli cell-only syndrome (SCOS). Intervention: Testicular samples of patients with the same histology were pooled for complementary DNA (cDNA) microarray analysis. Main Outcome Measure: Novel, down-regulated genes. Results: In total, 300 genes were significantly down-regulated in SCOS or MA samples, and 10 novel sterility-related genes were identified. Of the 10 novel genes, 6 genes (Hs.126780, Hs.553658, Hs.274135, Hs.268122, Hs.531701, and Hs.171130) encode proteins with predictable functional domains, and all these functional domains are believed to correlate with spermatogenesis and/or spermiogenesis. Conversely, the other 4 genes (Hs.351582, Hs.407480, Hs.552781, and Hs.355570) do not encompass known functional domains. Two genes (Hs.407480 and Hs.552781) lack mouse orthologues. Most novel genes showed a testis-specific expression pattern in both mice and humans. Reverse transcription-polymerase chain reaction (RT-PCR) showed three distinct types of developmental stage-dependent expressions of message ribonucleic acid (mRNA) for these novel genes in murine testes. Conclusion: These 10 novel genes provide targets to elucidate novel pathways involved in human spermatogenesis.
引用
收藏
页码:1650 / 1658
页数:9
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