Transmission bottlenecks and RNAi collectively influence tick-borne flavivirus evolution

被引：40
作者：
Grubaugh, Nathan D. [1 ,3 ]
Ruckert, Claudia [1 ]
Armstrong, Philip M. [2 ]
Bransfield, Angela [2 ]
Anderson, John F. [2 ]
Ebel, Gregory D. [1 ]
Brackney, Doug E. [2 ]
机构：
[1] Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO USA
[2] Ctr Vector Biol & Zoonot Dis, Connecticut Agr Expt Stn, New Haven, CT 06511 USA
[3] Scripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
来源：
VIRUS EVOLUTION | 2016年 / 2卷 / 02期
关键词：
flavivirus; evolution; Powassan virus; RNAi; Ixodes; tick; WEST-NILE-VIRUS; NATURALLY INFECTED MOSQUITOS; POWASSAN VIRUS; CHIKUNGUNYA VIRUS; IXODES-SCAPULARIS; MOLECULAR EPIDEMIOLOGY; ENCEPHALITIS-VIRUS; NEW-ENGLAND; HOST; INTERFERENCE;
D O I：
10.1093/ve/vew033
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Arthropod-borne RNA viruses exist within hosts as heterogeneous populations of viral variants and, as a result, possess great genetic plasticity. Understanding the micro-evolutionary forces shaping these viruses can provide insights into how they emerge, adapt, and persist in new and changing ecological niches. While considerable attention has been directed toward studying the population dynamics of mosquito-borne viruses, little is known about tick-borne virus populations. Therefore, using a mouse and Ixodes scapularis tick transmission model, we examined Powassan virus (POWV; Flaviviridae, Flavivirus) populations in and between both the vertebrate host and arthropod vector. We found that genetic bottlenecks, RNAi-mediated diversification, and selective constraints collectively influence POWV evolution. Together, our data provide a mechanistic explanation for the slow, long-term evolutionary trends of POWV, and suggest that all arthropod-borne viruses encounter similar selective pressures at the molecular level (i.e. RNAi), yet evolve much differently due to their unique rates and modes of transmission.
引用
收藏
页数：14
相关论文
共 82 条
  • [11] Stable Prevalence of Powassan Virus in Ixodes scapularis in a Northern Wisconsin Focus
    Brackney, Doug E.
    Nofchissey, Robert A.
    Fitzpatrick, Kelly A.
    Brown, Ivy K.
    Ebel, Gregory D.
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2008, 79 (06) : 971 - 973
  • [12] A single positively selected West Nile viral mutation confers increased virogenesis in American crows
    Brault, Aaron C.
    Huang, Claire Y-H
    Langevin, Stanley A.
    Kinney, Richard M.
    Bowen, Richard A.
    Ramey, Wanichaya N.
    Panella, Nicholas A.
    Holmes, Edward C.
    Powers, Ann M.
    Miller, Barry R.
    [J]. NATURE GENETICS, 2007, 39 (09) : 1162 - 1166
  • [13] FITNESS OF RNA VIRUS DECREASED BY MULLER RATCHET
    CHAO, L
    [J]. NATURE, 1990, 348 (6300) : 454 - 455
  • [14] Quantification of intrahost bottlenecks of West Nile virus in Culex pipiens mosquitoes using an artificial mutant swarm
    Ciota, Alexander T.
    Ehrbar, Dylan J.
    Van Slyke, Greta A.
    Payne, Anne F.
    Willsey, Graham G.
    Viscio, Rachael E.
    Kramer, Laura D.
    [J]. INFECTION GENETICS AND EVOLUTION, 2012, 12 (03) : 557 - 564
  • [15] Experimental transmission of Powassan virus (Flaviviridae) by Ixodes scapularis ticks (Acari:Ixodidae)
    Costero, A
    Grayson, MA
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1996, 55 (05) : 536 - 546
  • [16] RNA interference for the study and genetic manipulation of ticks
    de la Fuente, Jose
    Kocan, Katherine M.
    Almazan, Consuelo
    Blouin, Edmour F.
    [J]. TRENDS IN PARASITOLOGY, 2007, 23 (09) : 427 - 433
  • [17] Substantial biases in ultra-short read data sets from high-throughput DNA sequencing
    Dohm, Juliane C.
    Lottaz, Claudio
    Borodina, Tatiana
    Himmelbauer, Heinz
    [J]. NUCLEIC ACIDS RESEARCH, 2008, 36 (16)
  • [18] RAPID FITNESS LOSSES IN MAMMALIAN RNA VIRUS CLONES DUE TO MULLER RATCHET
    DUARTE, E
    CLARKE, D
    MOYA, A
    DOMINGO, E
    HOLLAND, J
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (13) : 6015 - 6019
  • [19] Short report: Duration of tick attachment required for transmission of Powassan virus by deer ticks
    Ebel, GD
    Kramer, LD
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2004, 71 (03) : 268 - 271
  • [20] Enzootic transmission of deer tick virus in New England and Wisconsin sites
    Ebel, GD
    Campbell, EN
    Goethert, HK
    Spielman, A
    Telford, SR
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2000, 63 (1-2) : 36 - 42
123456789