Novel Salt-Tolerant Xylanase from a Mangrove-Isolated Fungus Phoma sp. MF13 and Its Application in Chinese Steamed Bread

被引：22

作者：

Wu, JingJing ^{[1
]}

Qiu, Conghua ^{[1
]}

Ren, Yaxin ^{[1
]}

Yan, Renxiang ^{[1
]}

Ye, Xiuyun ^{[1
]}

Wang, Guozeng ^{[1
]}

机构：

[1] Fuzhou Univ, Fujian Key Lab Marine Enzyme Engn, 2 Xue Yuan Rd, Fuzhou 350116, Fujian, Peoples R China

来源：

ACS OMEGA | 2018年 / 3卷 / 04期

基金：

中国国家自然科学基金;

关键词：

ENDOPHYTIC FUNGI; CORN STOVER; MOLECULAR CHARACTERIZATION; HETEROLOGOUS EXPRESSION; AVICENNIA-MARINA; LIGNIN REMOVAL; DIVERSITY; GENE; SOIL; XYLOOLIGOSACCHARIDES;

D O I：

10.1021/acsomega.8b00345

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel glycosyl hydrolase family 11 xylanase gene, xynMF13A, was cloned from Phoma sp. MF13, a xylanase-producing fungus isolated from mangrove sediment. xynMF13A was heterologously expressed in Pichia pastoris, and the recombinant XynMF13A (rXynMF13A) was purified by Ni-affinity chromatography. The temperature and pH optima of purified rXynMF13A were 45 degrees C and pH 5.0, respectively. rXynMF13A showed a high level of salt tolerance, with maximal enzyme activity being seen at 0.5 M NaCl and as much as 53% of maximal activity at 4 M NaCl. The major rXynMF13A hydrolysis products from corncob xylan were xylobiose, xylotriose, xylotetraose, and xylopentaose, but no xylose was found. These hydrolysis products suggest an important potential for XynMF13A in the production of xylooligosaccharides (XOs). Furthermore, rXynMF13A had beneficial effects on Chinese steamed bread production, by increasing specific volume and elasticity while decreasing hardness and chewiness. These results demonstrate XynMF13A to be a novel xylanase with potentially significant applications in baking, XOs production, and seafood processing.

引用

页码：3708 / 3716

页数：9

共 55 条

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