Isolation, identification, and gp85 characterization of a subgroup A avian leukosis virus from a contaminated live Newcastle Disease virus vaccine, first report in China

被引:34
作者
Zhao, Peng [1 ]
Dong, Xuan [1 ]
Cui, Zhizhong [1 ]
机构
[1] Shandong Agr Univ, Coll Vet Med, Tai An, Shandong, Peoples R China
关键词
subgroup A of avian leukosis virus; gp85; gene; Newcastle disease virus; live vaccine; contamination; RETICULOENDOTHELIOSIS VIRUS; UNITED-STATES; CHICKENS; INFECTION; VARIANTS; ENVELOPE; STRAIN;
D O I
10.3382/ps.2014-03963
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
To identify if any exogenous avian leukosis virus (ALV) exists in a live vaccine of poultry according to the directives of the Ministry of Agriculture of the People's Republic of China, a live vaccine strain of the Newcastle disease virus (NDV) was neutralized using an anti-NDV antibody, and was subsequently used to inoculate DF-1 cells to investigate the presence of exogenous ALV. The DF-1 cells were cultured for 21 d and subsequently screened using an ELISA for the p27 antigen of the ALV. An exogenous ALV, designated ALV-NDVP4, was identified. The nucleotide sequence of the gp85 gene of the ALV-NDVP4 was compared with those of the various subgroups of the ALV. The amino acid sequence identities for the predicted gp85 of the ALV-NDVP4 and those of the ALV reference strains ranged from 88.2 to 99.5% for the 12 of the subgroup A strains of ALV (ALV-A) and from 82.7 to 87.4% for the B, C, D, and E subgroup strains. The amino acid sequence identities for the gp85 of the ALV-NDVP4 and those of the subgroup J reference strains ranged from 48.7 to 49.9%. The ALV-NDVP4 shared the highest level of homology with the SDAU09C3 strain of ALV-A, which was isolated in China, suggesting a common origin. This is the first report of ALV-A contamination in a live vaccine for poultry in China. Our findings highlight the need for improved monitoring methods for poultry vaccine production.
引用
收藏
页码:2168 / 2174
页数:7
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