GlnB is specifically required for Azospirillum brasilense NifA activity in Escherichia coli

被引：26

作者：

Araújo, LA ^{[1
]}

Monteiro, RA ^{[1
]}

Souza, EA ^{[1
]}

Steffens, ABR ^{[1
]}

Rigo, LU ^{[1
]}

Pedrosa, FO ^{[1
]}

Chubatsu, LS ^{[1
]}

机构：

[1] Univ Fed Parana, Dept Biochem & Mol Biol, BR-81531990 Curitiba, Parana, Brazil

来源：

RESEARCH IN MICROBIOLOGY | 2004年 / 155卷 / 06期

关键词：

Azospirillum brasilense; NifA; PII-like protein; GlnK; GInZ; GlnB; nitrogen fixation;

D O I：

10.1016/j.resmic.2004.03.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Azospirillum brasilense transcription regulator NifA and the nitrogen-status signaling proteins GlnB, GlnZ and GlnK were expressed in Escherichia coli and analyzed for their ability to activate nif gene expression. When expressed separately, none of the proteins were able to activate nifH promoter expression in any tested conditions; in contrast, nifH expression was observed in cells grown in the absence of ammonium and oxygen and when expressing simultaneously NifA and GlnB proteins, but not when expressing NifA and GlnZ or GlnK. Our results show that the GlnB protein is required for transcription activation by Azospirillum brasilense NifA and it cannot be replaced by GlnZ or GlnK. (C) 2004 Elsevier SAS. All rights reserved.

引用

页码：491 / 495

页数：5

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