Prevalence and Genetic Characterization of Cryptosporidium in Yaks in Qinghai Province of China

被引:47
作者
Mi, Rongsheng [1 ]
Wang, Xiaojuan [1 ]
Li, Chunhua [2 ]
Huang, Yan [1 ]
Zhou, Peng [1 ]
Li, Zhengfeng [1 ]
Lei, Mengtong [2 ]
Cai, Jinzhong [2 ]
Chen, Zhaoguo [1 ]
机构
[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Minist Agr, Key Lab Anim Parasitol, Shanghai, Peoples R China
[2] Qinghai Acad Anim & Vet Sci, Vet Res Inst, Lab Plateau Vet Parasitol, Xining, Peoples R China
来源
PLOS ONE | 2013年 / 8卷 / 09期
关键词
MOLECULAR CHARACTERIZATION; DAIRY CALVES; SUBTYPE ANALYSIS; SUBGENOTYPE ANALYSIS; CATTLE; HUMANS; PARVUM; GIARDIA; SPP; GENOTYPES;
D O I
10.1371/journal.pone.0074985
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The objective of this study was to determine the prevalence, species and subtypes of Cryptosporidium infecting yaks in the Qinghai Province of Northwestern China. The prevalence of Cryptosporidium spp. was detected by microscopy and nested-PCR. A total of 586 fecal samples were collected from yaks in 6 counties, of which 142 (24.2%) samples tested positive for Cryptosporidium. The small subunit (SSU) rRNA gene of fifty-five samples were amplified and sequenced successfully and demonstrated that Cryptosporidium bovis (31/55, 56.4%) was the most common species, followed by C. parvum (16/55, 29.1%) and C. ryanae (5/55, 9.0%). Mixed infections of C. parvum and C. bovis (n = 2), C. ryanae and C. bovis (n = 1) were also detected. All three species were found in yaks ranging in age from <1 year, 1-2 years, to >2 years. Cryptosporidium was most commonly detected in spring (28.4%), followed by summer (20.9%), then winter (17.5%). Cryptosporidium parvum positive samples were subtyped using the 60 kDa glycoprotein (gp60) gene. Subtypes IIaA15G2R1 (n = 8), IIaA16G2R1 (n = 2), IIaA14G1R1 (n = 1), IIaA14G2R1 (n = 1) and IIaA16G3R1 (n = 1) were detected. All of these subtypes are zoonotic, and may pose a potential threat to human health.
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