Flow injection spectrophotometric determination of L-ascorbic acid in biological matters

被引：31
作者：
Kleszczewski, T
Kleszczewska, E
机构：
[1] Med Acad, Inst Biophys, PL-15230 Bialystok, Poland
[2] Univ Bialystok, Inst Chem, PL-15443 Bialystok, Poland
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2002年 / 29卷 / 04期
关键词：
flow injection analysis; L-ascorbic acid; tissues;
D O I：
10.1016/S0731-7085(02)00127-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for spectrophotometric determination Of L-ascorbic acid (vitamin Q in tissues by flow injection analysis is reported. The procedure is based on the oxidation of analyse with iron(III) and 2,2'-dipyridyl. Concentrations of vitamin C in the range of 0.5-20 ppm have been determined with a relative standard deviation 1.2% (n = 15). The injection rate was 40 samples per h. The method was applied to the determination Of L-ascorbic acid in rat's tissues (blood serum, brain, and liver) and compared favourably with an independent reference method based on spectrophotometry. (C) 2002 Published by Elsevier Science B.V.
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收藏
页码：755 / 759
页数：5
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