Construction of a semisynthetic antibody library using trinucleotide oligos

被引：33

作者：

Braunagel, M ^{[1
]}

Little, M ^{[1
]}

机构：

[1] DEUTSCH KREBSFORSCHUNGSZENTRUM,DIV DIAG & EXPT THERAPY,RECOMBINANT ANTIBODY GRP,D-69120 HEIDELBERG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 22期

关键词：

D O I：

10.1093/nar/25.22.4690

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A semisynthetic antibody library composed of single a) chain Fv fragments (scFv) was constructed by replacing the heavy chain CDR3 region of a human scFv by a random sequence of eight amino acids using trinucleotide codons, After cloning into a phage display vector, an antibody library was generated with a complexity of 8 x 10(8) independent clones, The library was screened for binders to dinitrophenol, fluorescein isothiocyanate and 3-nitro-4-hydroxy-5-iodophenyl-acetic acid, scFv antibodies that specifically bound the antigen were obtained in each case.

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页码：4690 / 4691

页数：2

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